Analysis of fluorescently labeled oligosaccharides by capillary electrophoresis and electrospray ionization mass spectrometry

被引：9
|
作者：
Wolff, MW
Bazin, HG
Lindhardt, RJ
机构：
[1] Univ Iowa, Dept Chem, Div Med & Nat Prod Chem, Iowa City, IA 52242 USA
[2] Univ Iowa, Dept Chem & Biochem Engn, Iowa City, IA 52242 USA
来源：
BIOTECHNOLOGY TECHNIQUES | 1999年 / 13卷 / 11期
关键词：
electrospray mass spectrometry; exoglycosidase; neutral oligosaccharide; preparative capillary electrophoresis;
D O I：
10.1023/A:1008961114201
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Neutral oligosaccharides were fluorescently conjugated with 7-amino-1,3-naphthalenedisulfonic acid. A mixture of fluorescently labeled chitobiose, chitotriose, and chitotetrose were successfully separated by preparative capillary electrophoresis (CE) and the individual components characterized by electrospray ionization-mass spectrometry (ESI-MS). By combining fluorescent labeling with CE, the use of highly specific exoglycosidases and ESI-MS, a more structurally complex N-linked glycan was analyzed.
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页码：797 / 801
页数：5
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