Analysis of fluorescently labeled oligosaccharides by capillary electrophoresis and electrospray ionization mass spectrometry

被引：9
|
作者：
Wolff, MW
Bazin, HG
Lindhardt, RJ
机构：
[1] Univ Iowa, Dept Chem, Div Med & Nat Prod Chem, Iowa City, IA 52242 USA
[2] Univ Iowa, Dept Chem & Biochem Engn, Iowa City, IA 52242 USA
来源：
BIOTECHNOLOGY TECHNIQUES | 1999年 / 13卷 / 11期
关键词：
electrospray mass spectrometry; exoglycosidase; neutral oligosaccharide; preparative capillary electrophoresis;
D O I：
10.1023/A:1008961114201
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Neutral oligosaccharides were fluorescently conjugated with 7-amino-1,3-naphthalenedisulfonic acid. A mixture of fluorescently labeled chitobiose, chitotriose, and chitotetrose were successfully separated by preparative capillary electrophoresis (CE) and the individual components characterized by electrospray ionization-mass spectrometry (ESI-MS). By combining fluorescent labeling with CE, the use of highly specific exoglycosidases and ESI-MS, a more structurally complex N-linked glycan was analyzed.
引用
收藏
页码：797 / 801
页数：5
相关论文
共 50 条
  • [1] Analysis of heparin oligosaccharides by capillary electrophoresis-negative-ion electrospray ionization mass spectrometry
    Lin, Lei
    Liu, Xinyue
    Zhang, Fuming
    Chi, Lianli
    Amster, I. Jonathan
    Leach, Franklyn E., III
    Xia, Qiangwei
    Linhardt, Robert J.
    [J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2017, 409 (02) : 411 - 420
  • [2] Analysis of heparin oligosaccharides by capillary electrophoresis–negative-ion electrospray ionization mass spectrometry
    Lei Lin
    Xinyue Liu
    Fuming Zhang
    Lianli Chi
    I. Jonathan Amster
    Franklyn E. Leach
    Qiangwei Xia
    Robert J. Linhardt
    [J]. Analytical and Bioanalytical Chemistry, 2017, 409 : 411 - 420
  • [3] Analysis of metal species by using electrospray ionization mass spectrometry and capillary electrophoresis electrospray ionization mass spectrometry
    Schramel, O
    Michalke, B
    Kettrup, A
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1998, 819 (1-2) : 231 - 242
  • [4] Analysis of metal species by using electrospray ionization mass spectrometry and capillary electrophoresis-electrospray ionization mass spectrometry
    [J]. J Chromatogr, 1-2 (231-242):
  • [5] CAPILLARY ELECTROPHORESIS ELECTROSPRAY IONIZATION-MASS SPECTROMETRY
    EDMONDS, CG
    LOO, JA
    BARINAGA, CJ
    UDSETH, HR
    SMITH, RD
    [J]. JOURNAL OF CHROMATOGRAPHY, 1989, 474 (01): : 21 - 37
  • [6] Amino acid analysis by capillary electrophoresis electrospray ionization mass spectrometry
    Soga, T
    Heiger, DN
    [J]. ANALYTICAL CHEMISTRY, 2000, 72 (06) : 1236 - 1241
  • [7] Analysis of bisphosphonates by capillary electrophoresis-electrospray ionization mass spectrometry
    Huikko, K
    Kostiainen, R
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2000, 872 (1-2) : 289 - 298
  • [8] Applications of capillary electrophoresis electrospray ionization mass spectrometry in glycosaminoglycan analysis
    Zamfir, Alina D.
    [J]. ELECTROPHORESIS, 2016, 37 (7-8) : 973 - 986
  • [9] Amino acid analysis by capillary electrophoresis electrospray ionization mass spectrometry
    Soga, Tomoyoshi
    Heiger, David N.
    [J]. 2000, ACS, Washington, DC, USA (72)
  • [10] Analysis of inositol phosphate metabolism by capillary electrophoresis electrospray ionization mass spectrometry
    Qiu, Danye
    Wilson, Miranda S.
    Eisenbeis, Verena B.
    Harmel, Robert K.
    Riemer, Esther
    Haas, Thomas M.
    Wittwer, Christopher
    Jork, Nikolaus
    Gu, Chunfang
    Shears, Stephen B.
    Schaaf, Gabriel
    Kammerer, Bernd
    Fiedler, Dorothea
    Saiardi, Adolfo
    Jessen, Henning J.
    [J]. NATURE COMMUNICATIONS, 2020, 11 (01)
12345