Porous Silicon Biosensor for the Detection of Bacteria through Their Lysate

被引:11
|
作者
Vercauteren, Roselien [1 ]
Leprince, Audrey [2 ]
Mahillon, Jacques [2 ]
Francis, Laurent A. [1 ]
机构
[1] UCLouvain, Inst Informat & Commun Technol Elect & Appl Math, Dept Elect Engn, B-1348 Louvain La Neuve, Belgium
[2] UCLouvain, Lab Food & Environm Microbiol, Earth & Life Inst, B-1348 Louvain La Neuve, Belgium
来源
BIOSENSORS-BASEL | 2021年 / 11卷 / 02期
关键词
porous silicon membrane; bacterial detection; selective lysis; endolysins; lysostaphin; flow-through; FLOW-THROUGH; OPTICAL BIOSENSOR; RING-RESONATOR; SENSITIVITY; FABRICATION; MEMBRANES; STABILIZATION;
D O I
10.3390/bios11020027
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Porous silicon (PSi) has been widely used as a biosensor in recent years due to its large surface area and its optical properties. Most PSi biosensors consist in close-ended porous layers, and, because of the diffusion-limited infiltration of the analyte, they lack sensitivity and speed of response. In order to overcome these shortcomings, PSi membranes (PSiMs) have been fabricated using electrochemical etching and standard microfabrication techniques. In this work, PSiMs have been used for the optical detection of Bacillus cereus lysate. Before detection, the bacteria are selectively lysed by PlyB221, an endolysin encoded by the bacteriophage Deep-Blue targeting B. cereus. The detection relies on the infiltration of bacterial lysate inside the membrane, which induces a shift of the effective optical thickness. The biosensor was able to detect a B. cereus bacterial lysate, with an initial bacteria concentration of 10(5) colony forming units per mL (CFU/mL), in only 1 h. This proof-of-concept also illustrates the specificity of the lysis before detection. Not only does this detection platform enable the fast detection of bacteria, but the same technique can be extended to other bacteria using selective lysis, as demonstrated by the detection of Staphylococcus epidermidis, selectively lysed by lysostaphin.
引用
收藏
页数:15
相关论文
共 50 条
  • [31] Label-free quantitative detection using porous silicon as optical biosensor
    Zhong Fu-ru
    Ma Xiao-lin
    Lu Xiao-yi
    Jia Zhen-hong
    OPTOELECTRONICS LETTERS, 2012, 8 (04) : 314 - 317
  • [32] Detection of Echinococcus granulosus antigen by a quantum dot/porous silicon optical biosensor
    Li, Yanyu
    Jia, Zhenhong
    Lv, Guodong
    Wen, Hao
    Li, Peng
    Zhang, Hongyan
    Wang, Jiajia
    BIOMEDICAL OPTICS EXPRESS, 2017, 8 (07): : 3458 - 3469
  • [33] Label-free quantitative detection using porous silicon as optical biosensor
    钟福如
    马晓林
    吕小毅
    贾振红
    OptoelectronicsLetters, 2012, 8 (04) : 314 - 317
  • [34] Detection of Ammonia-Oxidizing Bacteria (AOB) Using a Porous Silicon Optical Biosensor Based on a Multilayered Double Bragg Mirror Structure
    Zhang, Hongyan
    Lv, Jie
    Jia, Zhenhong
    SENSORS, 2018, 18 (01):
  • [35] Protease detection using a porous silicon based Bloch surface wave optical biosensor
    Qiao, Hong
    Guan, Bin
    Gooding, J. Justin
    Reece, Peter J.
    OPTICS EXPRESS, 2010, 18 (14): : 15174 - 15182
  • [36] Porous silicon biosensor for detection of variable domain of heavy-chain of HCAb antibody
    张红燕
    吕小毅
    贾振红
    李江伟
    张富春
    Optoelectronics Letters, 2012, 8 (02) : 81 - 83
  • [37] Porous silicon biosensor for detection of variable domain of heavy-chain of HCAb antibody
    Hong-yan Zhang
    Xiao-yi Lü
    Zhen-hong Jia
    Jiang-wei Li
    Fu-chun Zhang
    Optoelectronics Letters, 2012, 8 (2) : 81 - 83
  • [38] Porous silicon biosensor for detection of variable domain of heavy-chain of HCAb antibody
    Zhang Hong-yan
    Lu Xiao-yi
    Jia Zhen-hong
    Li Jiang-wei
    Zhang Fu-chun
    OPTOELECTRONICS LETTERS, 2012, 8 (02) : 81 - 83
  • [39] A lectin-coupled porous silicon-based biosensor: label-free optical detection of bacteria in a real-time mode
    Yaghoubi, Mona
    Rahimi, Fereshteh
    Negahdari, Babak
    Rezayan, Ali Hossein
    Shafiekhani, Azizollah
    SCIENTIFIC REPORTS, 2020, 10 (01)
  • [40] A lectin-coupled porous silicon-based biosensor: label-free optical detection of bacteria in a real-time mode
    Mona Yaghoubi
    Fereshteh Rahimi
    Babak Negahdari
    Ali Hossein Rezayan
    Azizollah Shafiekhani
    Scientific Reports, 10