Establishment of neurofilament light chain Simoa assay in cerebrospinal fluid and blood

被引：84
|
作者：
Hendricks, Robert [1 ]
Baker, Dana [1 ]
Brumm, Jochen [2 ]
Davancaze, Teresa [1 ]
Harp, Chris [3 ]
Herman, Ann [3 ]
von Budingen, H-Christian [4 ]
Townsend, Michael [5 ]
Fischer, Saloumeh K. [1 ]
机构：
[1] Genentech Inc, Dept BioAnalyt Sci, San Francisco, CA 94080 USA
[2] Genentech Inc, Dept Biostat, San Francisco, CA 94080 USA
[3] Genentech Inc, OMNI Biomarker Dev, San Francisco, CA 94080 USA
[4] Roche, Neurosci, Prod Dev, Basel, Switzerland
[5] Genentech Inc, Biomarker Discovery OMNI, San Francisco, CA 94080 USA
来源：
BIOANALYSIS | 2019年 / 11卷 / 15期
关键词：
Alzheimer's disease (AD); amyotrophic lateral sclerosis (ALS); biomarker; cerebrospinal fluid (CSF); multiple sclerosis (MS); neurodegeneration; neurofilament light (NfL) chain; Quanterix Simoa (TM); UmanDiagnostics Nf-Light ELISA (Uman ELISA); QUANTIFICATION; BIOMARKER; SERUM;
D O I：
10.4155/bio-2019-0163
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Background: Neurofilament light (NfL) chain is an established cerebrospinal fluid (CSF) biomarker for neuroaxonal injury. The highly sensitive Quanterix SimoaT platform is evaluated for NfLmeasurement in both CSF and blood. There is a need to link historical ELISA data that use bovine NfL to that of Simoa using a recombinant human (rhuman) NfL standard. Results/Methodology: The Simoa NF-light (R) Advantage Kit was validated for CSF and qualified for serum and plasma, using both rhuman and bovine NfL calibrators. Matched CSF, serum and plasma samples from 112 multiple sclerosis patients were analyzed using both calibrators. Conclusion: In multiple sclerosis, there is a good correlation between blood and CSF NfL levels. A conversion factor of approximately 5:1 was established between bovine and rhuman NfL calibrators.
引用
收藏
页码：1405 / 1418
页数：14
相关论文
共 50 条
  • [31] ATN incorporating cerebrospinal fluid neurofilament light chain detects frontotemporal lobar degeneration
    Cousins, Katheryn A. Q.
    Phillips, Jeffrey S.
    Irwin, David J.
    Lee, Edward B.
    Wolk, David A.
    Shaw, Leslie M.
    Zetterberg, Henrik
    Blennow, Kaj
    Burke, Sarah E.
    Kinney, Nikolas G.
    Gibbons, Garrett S.
    McMillan, Corey T.
    Trojanowski, John Q.
    Grossman, Murray
    ALZHEIMERS & DEMENTIA, 2021, 17 (05) : 822 - 830
  • [32] Neurofilament light chain in cerebrospinal fluid or blood as a biomarker for mild cognitive impairment A systematic review and meta-analysis
    Zhang, Jing
    Cheng, Hongjiang
    Liu, Wei
    Li, Huimin
    Song, Yi
    Jia, Longbin
    MEDICINE, 2022, 101 (09) : E28932
  • [33] Cerebrospinal fluid and blood levels of neurofilament light chain in Parkinson disease A protocol for systematic review and meta-analysis
    Wang, HongZhou
    Wang, WanHua
    Shi, HaiCun
    Han, LiJian
    Pan, PingLei
    MEDICINE, 2020, 99 (31) : E21458
  • [34] Cerebrospinal fluid neurofilament light chain illustrates that semantic dementia is a distinctive neurodegenerative disease
    Meeter, L. H.
    Steketee, R.
    Salkovic, D.
    Vos, M.
    Grossman, M.
    McMillan, C.
    Boxer, A. L.
    Rojas-Martinez, J.
    Pijnenburg, Y. A.
    Sanchez-Valle, R.
    Diehl-Schmid, J.
    Santillo, A. F.
    Borroni, B.
    Galimberti, D.
    Rohrer, J.
    Synofzik, M.
    Mendonca, A.
    Vandenberghe, R.
    Benussi, L.
    Niessen, W.
    Kaat, L. Donker
    Teunissen, C.
    Bron, E.
    van Den Berg, E.
    van Swieten, J. C.
    EUROPEAN JOURNAL OF NEUROLOGY, 2018, 25 : 381 - 382
  • [35] Cerebrospinal fluid and blood neurofilament light chain levels in amyotrophic lateral sclerosis and frontotemporal degeneration: A meta-analysis
    Verde, Federico
    Licaj, Sara
    Soranna, Davide
    Ticozzi, Nicola
    Silani, Vincenzo
    Zambon, Antonella
    EUROPEAN JOURNAL OF NEUROLOGY, 2024, 31 (09)
  • [36] Comparison of SIMOA and VEUS technologies for serum neurofilament light chain measurement in multiple sclerosis
    Pelisek, Ondrej
    Kusnierova, Pavlina
    Hradilek, Pavel
    Horakova, Jana
    Svub, Krystof
    Siprova, Katerina
    Sobek, Ondrej
    Ganesh, Aravind
    Hanzlikova, Pavla
    Volny, Ondrej
    Revendova, Kamila Zondra
    MULTIPLE SCLEROSIS AND RELATED DISORDERS, 2024, 90
  • [37] Cerebrospinal Fluid and Blood Neurofilament Light Chain Protein in Prion Disease and Other Rapidly Progressive Dementias: Current State of the Art
    Abu-Rumeileh, Samir
    Parchi, Piero
    FRONTIERS IN NEUROSCIENCE, 2021, 15
  • [38] Increased cerebrospinal fluid neurofilament light chain in central nervous system inflammatory demyelinating disease
    Peng, Lijun
    Bi, Chongfeng
    Xia, Deyu
    Mao, Linling
    Qian, Hairong
    MULTIPLE SCLEROSIS AND RELATED DISORDERS, 2019, 30 : 123 - 128
  • [39] Biological Sex Influences The Relationship Between Aortic Stiffness And Cerebrospinal Fluid Neurofilament Light Chain
    Corkery, Adam T.
    Pearson, Andrew G.
    Loggie, Nicole A.
    Miller, Kathleen B.
    Howery, Anna J.
    Johnson, Sterling C.
    Fico, Brandon G.
    Barnes, Jill N.
    MEDICINE & SCIENCE IN SPORTS & EXERCISE, 2024, 56 (10) : 53 - 53
  • [40] Cerebrospinal fluid neurofilament light chain levels: marker of progression to generalized amyotrophic lateral sclerosis
    Tortelli, R.
    Copetti, M.
    Ruggieri, M.
    Cortese, R.
    Capozzo, R.
    Leo, A.
    D'Errico, E.
    Mastrapasqua, M.
    Zoccolella, S.
    Pellegrini, F.
    Simone, I. L.
    Logroscino, G.
    EUROPEAN JOURNAL OF NEUROLOGY, 2015, 22 (01) : 215 - 218
12345