Establishment of neurofilament light chain Simoa assay in cerebrospinal fluid and blood

被引：84

作者：

Hendricks, Robert ^{[1
]}

Baker, Dana ^{[1
]}

Brumm, Jochen ^{[2
]}

Davancaze, Teresa ^{[1
]}

Harp, Chris ^{[3
]}

Herman, Ann ^{[3
]}

von Budingen, H-Christian ^{[4
]}

Townsend, Michael ^{[5
]}

Fischer, Saloumeh K. ^{[1
]}

机构：

[1] Genentech Inc, Dept BioAnalyt Sci, San Francisco, CA 94080 USA

[2] Genentech Inc, Dept Biostat, San Francisco, CA 94080 USA

[3] Genentech Inc, OMNI Biomarker Dev, San Francisco, CA 94080 USA

[4] Roche, Neurosci, Prod Dev, Basel, Switzerland

[5] Genentech Inc, Biomarker Discovery OMNI, San Francisco, CA 94080 USA

来源：

BIOANALYSIS | 2019年 / 11卷 / 15期

关键词：

Alzheimer's disease (AD); amyotrophic lateral sclerosis (ALS); biomarker; cerebrospinal fluid (CSF); multiple sclerosis (MS); neurodegeneration; neurofilament light (NfL) chain; Quanterix Simoa (TM); UmanDiagnostics Nf-Light ELISA (Uman ELISA); QUANTIFICATION; BIOMARKER; SERUM;

D O I：

10.4155/bio-2019-0163

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: Neurofilament light (NfL) chain is an established cerebrospinal fluid (CSF) biomarker for neuroaxonal injury. The highly sensitive Quanterix SimoaT platform is evaluated for NfLmeasurement in both CSF and blood. There is a need to link historical ELISA data that use bovine NfL to that of Simoa using a recombinant human (rhuman) NfL standard. Results/Methodology: The Simoa NF-light (R) Advantage Kit was validated for CSF and qualified for serum and plasma, using both rhuman and bovine NfL calibrators. Matched CSF, serum and plasma samples from 112 multiple sclerosis patients were analyzed using both calibrators. Conclusion: In multiple sclerosis, there is a good correlation between blood and CSF NfL levels. A conversion factor of approximately 5:1 was established between bovine and rhuman NfL calibrators.

引用

页码：1405 / 1418

页数：14

共 50 条

[1] Establishment of Neurofilament Light Chain (NfL) SIMOA assay as well as a conversion factor to enable comparison to historical results using Bovine calibrators
Hendricks, Robert
Davancaze, Teresa
Harp, Christopher
Herman, Ann
Baker, Dana
Brumm, Jochen
Von Budingen, H.
Townsend, Mike
Eggers, Erica
Fischer, Sally
NEUROLOGY, 2019, 92 (15)
[2] Neurofilament Light Chain Levels in Cerebrospinal Fluid and Plasma in Neurosarcoidosis
Byg, Keld-Erik
Sejbaek, Tobias
Nielsen, Helle
Illes, Zsolt
Madsen, Jonna Skov
Olsen, Dorte
Ellingsen, Torkell
ARTHRITIS & RHEUMATOLOGY, 2019, 71
[3] An enzyme immunoassay to quantify neurofilament light chain in cerebrospinal fluid
Van Geel, WJA
Rosengren, LE
Verbeek, MM
JOURNAL OF IMMUNOLOGICAL METHODS, 2005, 296 (1-2) : 179 - 185
[4] Guidance for use of neurofilament light chain as a cerebrospinal fluid and blood biomarker in multiple sclerosis management
Freedman, Mark S.
Gnanapavan, Sharmilee
Booth, Ronald A.
Calabresi, Peter A.
Khalil, Michael
Kuhle, Jens
Lycke, Jan
Olsson, Tomas
EBIOMEDICINE, 2024, 101
[5] Cerebrospinal Fluid and Serum Neurofilament Light Chain Levels in ALS Patients
Simone, Isabella L.
Ruggieri, Maddalena
Tortelli, Rosanna
D'Errico, Eustachio
Leo, Antonio
Mastrapasqua, Mariangela
Leante, Rosaria
Capozzo, Rosa
Livrea, Paolo
Logroscino, Giancarlo
NEUROLOGY, 2010, 74 (09) : A179 - A180
[6] A Case of HaNDL with Low Cerebrospinal Fluid Level of Neurofilament Light Chain
Sisman, Aysel Busra
Bayar, Muhammet Duran
Icoz, Sema
Yilmaz, Vuslat
Kurtuncu, Murat
Tuzun, Erdem
Ertas, Mustafa
Bastan, Birgul
CASE REPORTS IN NEUROLOGY, 2020, 12 (03) : 334 - 338
[7] Neurofilament light chain is a cerebrospinal fluid biomarker in hereditary spastic paraplegia
Kessler, Christoph
Serna-Higuita, Lina M.
Rattay, Tim W.
Maetzler, Walter
Wurster, Isabel
Hayer, Stefanie
Wilke, Carlo
Hengel, Holger
Reichbauer, Jennifer
Armbruster, Marcel
Schoels, Ludger
Martus, Peter
Schuele, Rebecca
ANNALS OF CLINICAL AND TRANSLATIONAL NEUROLOGY, 2021, 8 (05): : 1122 - 1131
[8] Clinical value of cerebrospinal fluid neurofilament light chain in semantic dementia
Meeter, Lieke H. H.
Steketee, Rebecca M. E.
Salkovic, Dina
Vos, Maartje E.
Grossman, Murray
McMillan, Corey T.
Irwin, David J.
Boxer, Adam L.
Rojas, Julio C.
Olney, Nicholas T.
Karydas, Anna
Miller, Bruce L.
Pijnenburg, Yolande A. L.
Barkhof, Frederik
Sanchez-Valle, Raquel
Llado, Albert
Borrego-Ecija, Sergi
Diehl-Schmid, Janine
Grimmer, Timo
Goldhardt, Oliver
Santillo, Alexander F.
Hansson, Oskar
Vestberg, Susanne
Borroni, Barbara
Padovani, Alessandro
Galimberti, Daniela
Scarpini, Elio
Rohrer, Jonathan D.
Woollacott, Ione O. C.
Synofzik, Matthis
Wilke, Carlo
de Mendonca, Alexandre
Vandenberghe, Rik
Benussi, Luisa
Ghidoni, Roberta
Binetti, Giuliano
Niessen, Wiro J.
Papma, Janne M.
Seelaar, Harro
Jiskoot, Lize C.
de Jong, Frank Jan
Kaat, Laura Donker
Del Campo, Marta
Teunissen, Charlotte E.
Bron, Esther E.
Van den Berg, Esther
Van Swieten, John C.
JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY, 2019, 90 (09): : 997 - 1004
[9] Evaluation of neurofilament light chain in the cerebrospinal fluid and blood as a biomarker for neuronal damage in experimental pneumococcal meningitis
Ngoc Dung Le
Lukas Muri
Denis Grandgirard
Jens Kuhle
David Leppert
Stephen L. Leib
Journal of Neuroinflammation, 17
[10] Evaluation of neurofilament light chain in the cerebrospinal fluid and blood as a biomarker for neuronal damage in experimental pneumococcal meningitis
Le, Ngoc Dung
Muri, Lukas
Grandgirard, Denis
Kuhle, Jens
Leppert, David
Leib, Stephen L.
JOURNAL OF NEUROINFLAMMATION, 2020, 17 (01)

← 1 2 3 4 5 →