Molecular cloning and evolution of the gene encoding the precursor of Nilgai (Boselaphus tragocamelus) interleukin 2

被引：2
|
作者：
Das, Dhanjit Kumar
Saini, Mohini [1 ]
Swarup, Devendra
Gupta, Praveen Kumar
机构：
[1] Indian Vet Res Inst, Ctr Wildlife, Izatnagar 243122, Uttar Pradesh, India
[2] Indian Vet Res Inst, Div Vet Biotechnol, Izatnagar 243122, Uttar Pradesh, India
来源：
DNA SEQUENCE | 2006年 / 17卷 / 06期
关键词：
boselaphus tragocamelus; cDNA sequence; interleukin-2; cloning;
D O I：
10.1080/10425170600908542
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Interleukin 2 is a key immunoregulatory cytokine that is involved in T helper differentiation and is known to induce interferon gamma (IFN-gamma) in natural killer (NK) and T-cell. The gene for interleukin-2 (IL-2) was amplified from cDNA pool prepared from ConAstimulated peripheral blood mononuclear cells (PBM cells) isolated from Nilgai reared in semicaptivity. The amplified IL-2 gene was cloned and nucleotide sequences were determined. The sequencing result showed that Nilgai IL-2 cDNA contains 468 bp long ORF encoding 155 amino acids (Genbank Accession No DQ017831). Homology comparison of nucleotide revealed that Nilgai IL-2 sequence is evolutionary more related to buffalo than cattle IL-2 sequence.
引用
收藏
页码：465 / 470
页数：6
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