Complementation analyses of Sinorhizobium meliloti nifA mutant with different originated nifA genes

被引:3
|
作者
Yao Zhenhua
Tian Zhexian
Dai Xiaomi
Becker, Anke
Li Jian
Yan Haiqin
Xiao Yan
Zhu Jiabi
Yu Guanqiao
Rueverg, Silvia
Wang Yiping
Zou Huasong [1 ]
机构
[1] Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China
[2] Peking Univ, Coll Life Sci, Natl Lab Prot Engn & Plant Genet Engn, Beijing 100871, Peoples R China
[3] Univ Bielefeld, Lehrstuhl Genet, D-33615 Bielefeld, Germany
[4] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
来源
CHINESE SCIENCE BULLETIN | 2006年 / 51卷 / 22期
关键词
Sinorhizobium meliloti; NifA; gene expression; microarray;
D O I
10.1007/s11434-006-2203-0
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
A previous work inferred that the nifA gene of Enterobacter cloacae did not restore the symbiotic phenotype of Sinorhizobium meliloti nifA mutant. In the present study, two nifA genes of Bradyrhizobiurn japonicum and Mesorhizobium huakuii also did not restore the symbiotic phenotype of S. meliloti nifA mutant. In whole genomic microarray experiments, 238 genes were found to be differentially expressed after S. meliloti nifA had been constitutively expressed in its nifA mutant. In contrast, only 20, 7 and 9 genes changed their transcriptional levels when expressing B. japonium, M. huakuii and Enterobacter cloacae nifA genes in Sm nifA mutant separately. These genes were classified into severai functional groups including house keeping, energy and central intermediary metabolism, transport systems and symbiosis. Interestingly, the genes that of nifH operons showed high expression levels in the presence of either B. japonium or M. huakuii NifA, which was confirmed by subsequent lacZ fusion experiments.
引用
收藏
页码:2748 / 2754
页数:7
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