A new cell line from larval fat bodies of the bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae)

被引:0
|
作者
Zhang, Huan
Zhang, Yong-An
Qin, Qilian
Wang, Yuzhu
Li, Xuan
Miao, Lin
Yin, Zhenxian
Zhang, Aijun
Qu, Liangjian
Ding, Cui
机构
[1] Chinese Acad Sci, Inst Zool, State Key Lab Integrated Management Pest Insects, Beijing 100080, Peoples R China
[2] Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, Key Lab Forest Protect State Forestry Adm, Beijing 100091, Peoples R China
关键词
Helicoverpa armigera; fat body; cell line; DAF-PCR; karyotype;
D O I
10.1290/0605033.1
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
A new cell line, designated IOZCAS-Ha-I, was initiated from the fat body of larvae of Helicoverpa armigera (Lepidoptera: Noctuidae) in TNM-FH medium containing 10% fetal bovine serum. Spherical cells were predominant among the various cell types. The cell line showed a typical lepidopteran chromosome pattern ranging from 58 to 239 chromosomes in the majority of the cells. It was confirmed to have originated from the H. armigera by the DNA amplification-fingerprinting polymerase chain reaction (DAF-PCR) technique. The new cell line was only slightly susceptible to the multiple nucleocapsid nuclear polyhedrosis viruses (NPV) from H. armigera.
引用
收藏
页码:290 / 293
页数:4
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