Protocol for rapid manipulation of mitochondrial morphology in living cells using inducible counter mitochondrial morphology (iCMM)

被引：0

作者：

Miyamoto, Takafumi ^{[1
,2
]}

Han, Song-Iee ^{[1
]}

Shimano, Hitoshi ^{[1
]}

机构：

[1] Univ Tsukuba, Fac Med, Dept Internal Med Endocrinol & Metab, Tsukuba, Ibaraki 3058575, Japan

[2] Univ Tsukuba, Transborder Med Res Ctr, Tsukuba, Ibaraki 3058577, Japan

来源：

STAR PROTOCOLS | 2021年 / 2卷 / 03期

关键词：

Biotechnology and bioengineering; Cell Biology; Molecular/Chemical Probes; Protein Biochemistry;

D O I：

10.1016/j.xpro.2021.100721

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Disruption of mitochondrial morphology occurs during various diseases, but the biological significance is not entirely clear. Here, we describe a detailed step-bystep protocol for a chemically inducible dimerization system-based synthetic protein device, termed inducible counter mitochondrial morphology. This system allows artificial manipulation of mitochondrial morphology on a timescale of minutes in living mammalian cells. We also describe an AI-assisted imaging processing approach.For complete details on the use and execution of this protocol, please refer to Miyamoto et al., 2021.

引用

页数：17

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