Protocol for rapid manipulation of mitochondrial morphology in living cells using inducible counter mitochondrial morphology (iCMM)

被引：0
|
作者：
Miyamoto, Takafumi [1 ,2 ]
Han, Song-Iee [1 ]
Shimano, Hitoshi [1 ]
机构：
[1] Univ Tsukuba, Fac Med, Dept Internal Med Endocrinol & Metab, Tsukuba, Ibaraki 3058575, Japan
[2] Univ Tsukuba, Transborder Med Res Ctr, Tsukuba, Ibaraki 3058577, Japan
来源：
STAR PROTOCOLS | 2021年 / 2卷 / 03期
关键词：
Biotechnology and bioengineering; Cell Biology; Molecular/Chemical Probes; Protein Biochemistry;
D O I：
10.1016/j.xpro.2021.100721
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Disruption of mitochondrial morphology occurs during various diseases, but the biological significance is not entirely clear. Here, we describe a detailed step-bystep protocol for a chemically inducible dimerization system-based synthetic protein device, termed inducible counter mitochondrial morphology. This system allows artificial manipulation of mitochondrial morphology on a timescale of minutes in living mammalian cells. We also describe an AI-assisted imaging processing approach.For complete details on the use and execution of this protocol, please refer to Miyamoto et al., 2021.
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页数：17
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