Characterization of outer membrane efflux proteins OpmE, OpmD and OpmB of Pseudomonas aeruginosa:: molecular cloning and development of specific antisera

被引：22

作者：

Murata, T ^{[1
]}

Gotoh, N ^{[1
]}

Nishino, T ^{[1
]}

机构：

[1] Kyoto Pharmaceut Univ, Dept Microbiol, Kyoto 6078414, Japan

来源：

FEMS MICROBIOLOGY LETTERS | 2002年 / 217卷 / 01期

关键词：

Pseudomonas aeruginosa; outer membrane protein; multidrug efflux system; ethidium accumulation; polyclonal antiserum;

D O I：

10.1111/j.1574-6968.2002.tb11456.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The third genes, opmE, opmD and opmB, of multidrug efflux operons deduced from the Pseudomonas aeruginosa PAO1 genome data were cloned by polymerase chain reaction. The opmB gene product showed functional cooperation with inner membrane-associated components, MexAB, MexCD and MexXY, of the previously characterized multidrug efflux systems responsible for resistance to antimicrobial agents and extrusion of ethidium. The opmE and opmD gene products did not show functional cooperation. Immunoblots using a specific rabbit antiserum demonstrated, through exponential to stationary phases, constant expression of opmB and growth phase-dependent expression of opmD. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：57 / 63

页数：7