Single-Cell Infrared Microspectroscopy Quantifies Dynamic Heterogeneity of Mesenchymal Stem Cells during Adipogenic Differentiation

被引：23
|
作者：
Wang, Yadi [1 ,2 ,3 ]
Dai, Wentao [4 ,5 ]
Liu, Zhixiao [2 ]
Liu, Jixiang [5 ]
Cheng, Jie [1 ,2 ]
Li, Yuanyuan [4 ,5 ]
Li, Xueling [6 ]
Hu, Jun [1 ,2 ]
Lu, Junhong [1 ,2 ]
机构：
[1] Chinese Acad Sci, Shanghai Adv Res Inst, Zhangjiang Lab, Shanghai Synchrotron Radiat Facil, Shanghai 201203, Peoples R China
[2] Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
[3] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
[4] Shanghai Jiao Tong Univ, Ruijin Hosp, Shanghai Inst Digest Surg, Shanghai Key Lab Gastr Neoplasms,Sch Med, Shanghai 200025, Peoples R China
[5] Shanghai Ctr Bioinformat Technol, Shanghai 201203, Peoples R China
[6] Shanghai Univ Med & Hlth Sci, Natl Engn Res Ctr Nanotechnol, Shanghai 201318, Peoples R China
来源：
ANALYTICAL CHEMISTRY | 2021年 / 93卷 / 02期
基金：
国家重点研发计划; 中国国家自然科学基金;
关键词：
32;
D O I：
10.1021/acs.analchem.0c04110
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The central relevance of cellular heterogeneity to biological phenomena raises the rational needs for analytical techniques with single-cell resolution. Here, we developed a single-cell FTIR microspectroscopy-based method for the quantitative evaluation of cellular heterogeneity by calculating the cell-to-cell similarity distance of the infrared spectral data. Based on this method, we revealed the infrared phenotypes might reflect the dynamic heterogeneity changes in the cell population during the adipogenic differentiation of the human mesenchymal stem cells. These findings provide an alternative label-free optical approach for quantifying the cellular heterogeneity, and the combination with other single-cell analysis tools will be very helpful for understanding the genotype-to-phenotype relationship in cellular populations.
引用
收藏
页码：671 / 676
页数：6
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