Global Analysis of Short RNAs Reveals Widespread Promoter-Proximal Stalling and Arrest of Pol II in Drosophila

被引：330

作者：

Nechaev, Sergei ^{[1
]}

Fargo, David C. ^{[2
,3
]}

dos Santos, Gilberto ^{[1
]}

Liu, Liwen ^{[4
]}

Gao, Yuan ^{[5
]}

Adelman, Karen ^{[1
]}

机构：

[1] NIEHS, Mol Carcinogenesis Lab, NIH, Res Triangle Pk, NC 27709 USA

[2] NIEHS, Lib Serv, NIH, Res Triangle Pk, NC 27709 USA

[3] NIEHS, Informat Serv, NIH, Res Triangle Pk, NC 27709 USA

[4] NIEHS, Biostat Branch, NIH, Res Triangle Pk, NC 27709 USA

[5] Virginia Commonwealth Univ, Dept Comp Sci, Richmond, VA 23284 USA

来源：

SCIENCE | 2010年 / 327卷 / 5963期

关键词：

CLEAVAGE FACTOR TFIIS; POLYMERASE-II; TRANSCRIPTION INITIATION; ELONGATION; MELANOGASTER; GENES; NELF; EXPRESSION; STABILITY; INVITRO;

D O I：

10.1126/science.1181421

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Emerging evidence indicates that gene expression in higher organisms is regulated by RNA polymerase II stalling during early transcription elongation. To probe the mechanisms responsible for this regulation, we developed methods to isolate and characterize short RNAs derived from stalled RNA polymerase II in Drosophila cells. Significant levels of these short RNAs were generated from more than one-third of all genes, indicating that promoter-proximal stalling is a general feature of early polymerase elongation. Nucleotide composition of the initially transcribed sequence played an important role in promoting transcriptional stalling by rendering polymerase elongation complexes highly susceptible to backtracking and arrest. These results indicate that the intrinsic efficiency of early elongation can greatly affect gene expression.

引用

页码：335 / 338

页数：4