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Wood profiling by non-targeted high-resolution mass spectrometry: Part 1, Metabolite profiling in Cedrela wood for the determination of the geographical origin
被引:10
|作者:
Creydt, Marina
[1
,2
]
Ludwig, Lea
[1
]
Kohl, Michael
[3
]
Fromm, Jorg
[2
,4
]
Fischer, Markus
[1
,2
]
机构:
[1] Univ Hamburg, Inst Food Chem, Hamburg Sch Food Sci, Grindelallee 117, D-20146 Hamburg, Germany
[2] Univ Hamburg, Cluster Excellence, Understanding Written Artefacts, Warburgstr 26, D-20354 Hamburg, Germany
[3] Univ Hamburg, Inst Wood Sci, Res Unit World Forestry, Leuschnerstr 91e, D-21031 Hamburg, Germany
[4] Univ Hamburg, Inst Wood Sci, Res Unit Wood Biol, Leuschnerstr 91e, D-21031 Hamburg, Germany
关键词:
non-targeted mass spectrometry;
metabolomics;
extraction;
wood;
illegal logging;
geographical origin;
TOTAL LIPID EXTRACTION;
SAMPLE PREPARATION;
STRESS TOLERANCE;
SALICYLIC-ACID;
PLANT;
METABOLOMICS;
CERAMIDE;
CHROMATOGRAPHY;
APOPTOSIS;
RESPONSES;
D O I:
10.1016/j.chroma.2021.461993
中图分类号:
Q5 [生物化学];
学科分类号:
071010 ;
081704 ;
摘要:
The determination of the geographical origin of wood can be highly relevant for several reasons: On the one hand, it can help to prevent illegal logging and timber trade, on the other hand, it is of special interest for archaeological artefacts made of wood, as well as for a variety of biological questions. For this reason, different extraction methods were first tested for the analysis of polar and non-polar metabolites using liquid chromatography coupled electrospray ionization quadrupole time-of-flight mass spectrometry (UHPLC-ESI-QTOF-MS). A two-phase extraction with chloroform, methanol and water proved to be particularly successful. Subsequently, cedrela (Cedrela odorata) samples from South America were measured to distinguish geographic origin. Using multivariate data analysis, numerous origin-dependent differences could be extracted. The identification of the marker substances indicated that several metabolic pathways were affected by the geographical influences, some of them probably indicating pest infections. ? 2021 Elsevier B.V. All rights reserved.
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页数:12
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