Protein Engineering of Carboxyl Esterases by Rational Design and Directed Evolution

被引：0
|
作者：
Schmidt, M. [1 ]
Boettcher, D. [1 ]
Bornscheuer, U. T. [1 ]
机构：
[1] Ernst Moritz Arndt Univ Greifswald, Inst Biochem, Dept Biotechnol & Enzyme Catalysis, D-17487 Greifswald, Germany
来源：
PROTEIN AND PEPTIDE LETTERS | 2009年 / 16卷 / 10期
关键词：
ITERATIVE SATURATION MUTAGENESIS; THROUGHPUT-SCREENING METHOD; TERTIARY ALCOHOLS; BACILLUS-SUBTILIS; PSEUDOMONAS-FLUORESCENS; ENANTIOSELECTIVE HYDROLASES; FUNCTIONAL EXPRESSION; BURKHOLDERIA-GLADIOLI; HYDROLYTIC ENZYMES; KINETIC RESOLUTION;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In the past few years a considerable number of mutagenesis methods and high-throughput screening (HTS) systems have been developed and improved. In parallel, computer programs or software packages for molecular modeling have been further investigated. Thus, the number of examples for successful directed evolution and rational design is increasing constantly. In this review the essential mutagenesis methods and HTS systems, especially for esterases, are described and various examples for the application of these protein engineering tools are provided.
引用
收藏
页码：1162 / 1171
页数：10
相关论文
共 50 条
  • [11] Engineering proteins for thermostability: the use of sequence alignments versus rational design and directed evolution
    Lehmann, M
    Wyss, M
    [J]. CURRENT OPINION IN BIOTECHNOLOGY, 2001, 12 (04) : 371 - 375
  • [12] Enzyme engineering: rational redesign versus directed evolution
    Chen, RD
    [J]. TRENDS IN BIOTECHNOLOGY, 2001, 19 (01) : 13 - 14
  • [13] Directed evolution: the 'rational' basis for 'irrational' design
    Tobin, MB
    Gustafsson, C
    Huisman, GW
    [J]. CURRENT OPINION IN STRUCTURAL BIOLOGY, 2000, 10 (04) : 421 - 427
  • [14] Protein engineering design from directed evolution to de novo synthesis
    Xiong, Wei
    Liu, Bo
    Shen, Yujiao
    Jing, Keju
    Savage, Thomas R.
    [J]. BIOCHEMICAL ENGINEERING JOURNAL, 2021, 174
  • [15] Directed evolution approaches for protein engineering
    Farinas, ET
    [J]. COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING, 2006, 9 (04) : 235 - 236
  • [16] Protein design by directed evolution
    Jaeckel, Christian
    Kast, Peter
    Hilvert, Donald
    [J]. ANNUAL REVIEW OF BIOPHYSICS, 2008, 37 : 153 - 173
  • [17] Engineering a Thermostable Keto Acid Decarboxylase Using Directed Evolution and Computationally Directed Protein Design
    Soh, Lemuel M. J.
    Mak, Wai Shun
    Lin, Paul P.
    Mi, Luo
    Chen, Frederic Y. -H.
    Damoiseaux, Robert
    Siegel, Justin B.
    Liao, James C.
    [J]. ACS SYNTHETIC BIOLOGY, 2017, 6 (04): : 610 - 618
  • [18] Rational design and engineering of an immunomodulatory protein
    Bogani, F
    Joshi, L
    Edupuganti, V
    Ganta, S
    Ghirlanda, G
    [J]. PROTEIN SCIENCE, 2004, 13 : 213 - 213
  • [19] Computational Protein Engineering: Bridging the Gap between Rational Design and Laboratory Evolution
    Barrozo, Alexandre
    Borstnar, Rok
    Marloie, Gael
    Kamerlin, Shina Caroline Lynn
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2012, 13 (10): : 12428 - 12460
  • [20] Engineering and Directed Evolution of Cellular Protein Translation
    Badran, Ahmed
    [J]. PROTEIN SCIENCE, 2021, 30 : 57 - 57
12345