Structure Control of Polysaccharide Derivatives for Efficient Separation of Enantiomers by Chromatography

The development and applications of polysaccharide derivatives for the efficient chromatographic separation of enantiomers have been investigated. Direct chiral separation using chiral stationary phases (CSPs) for high-performance liquid chromatography (HPLC) has significantly evolved and has been recognized as the most popular and reliable tool for both the analysis of enantiomer compositions. One of the advantages of this method is that the association constant between enantiomers and chiral selectors can be estimated by changing the concentration of chiral selectors in mobile phases. Cellulose phenylcarbamate derivatives are also useful chiral selectors for HPLC when coated on silica gel. Monolithic silica gel supports have attracted significant attention as a supporting material for a rapid chromatographic analysis, because the pressure drop through monolithic silica gel supports is significantly lower and the column efficiency on the linear flow rate of the mobile phase is much flatter compared with the spherical silica supports.