Influence of acid stress on survival, expression of virulence genes and invasion capacity into Caco-2 cells of Listeria monocytogenes strains of different origins

被引：42
|
作者：
Werbrouck, Hadewig [1 ]
Vermeulen, An [2 ]
Van Coillie, Els [1 ]
Messens, Winy [1 ]
Herman, Lieve [1 ]
Devlieghere, Frank [2 ]
Uyttendaele, Mieke [2 ]
机构：
[1] Flemish Govt, Inst Agr & Fisheries Res ILVO, Technol & Food Sci Unit, B-9090 Melle, Belgium
[2] Univ Ghent, Fac Biosci Engn, Lab Food Microbiol & Food Preservat, Dept Food Safety & Food Qual, B-9000 Ghent, Belgium
来源：
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 2009年 / 134卷 / 1-2期
关键词：
L; monocytogenes; Acid stress response; Expression; Virulence genes; Invasion; Caco-2; cells; BILE-SALT HYDROLASE; GLUTAMATE-DECARBOXYLASE; TOLERANCE RESPONSE; LOW PH; SIGMA(B); FOOD; PRFA; IDENTIFICATION; ADAPTATION; PRODUCTS;
D O I：
10.1016/j.ijfoodmicro.2009.03.022
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The influence of food-related acid stress on the virulence capacity of Listeria monocytogenes was evaluated. The survival of acid-adapted and non-adapted L. monocytogenes cells during exposure to lethal concentrations of acetic acid was monitored. Also the effect of sublethal acid stress exposure on the expression levels of several virulence genes and on the capacity to invade Caco-2 cells was analyzed. Acid-adapted and non-adapted cells showed different acid tolerance response (ATR) patterns. Our data also demonstrate that pre-exposure to sublethal acid stress might lead to gadD2 induction. However, no correlation with the origin of the strain and with the ATR was noticed, indicating that probably other genes also could play an important role in this ATR mechanism. Additionally, our data showed that acid adaptation could influence the virulence capacity by regulating the expression levels of virulence genes. Moreover, the inlA expression data strongly correlated with the results of the in vitro invasion study. These results lead to the indication that low pH and acetic acid. used in minimally processed food products, might influence the virulence potential of L. monocytogenes. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码：140 / 146
页数：7
相关论文
共 36 条
  • [21] Use of PCR-Restriction fragment length polymorphism of inlA for rapid screening of Listeria monocytogenes strains deficient in the ability to invade caco-2 cells
    Rousseaux, S
    Olier, M
    Lemaître, JP
    Piveteau, P
    Guzzo, J
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (04) : 2180 - 2185
  • [22] Exposure to salt and organic acids increases the ability of Listeria monocytogenes to invade Caco-2 cells but decreases its ability to survive gastric stress
    Garner, Matthew R.
    James, Karen E.
    Callahan, Michelle C.
    Wiedmann, Martin
    Boor, Kathryn J.
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (08) : 5384 - 5395
  • [23] IN VITRO ANTI-ADHERENT ASSESSMENT OF SELECTED LACTIC ACID BACTERIA ISOLATES AGAINST SALMONELLA TYPHIMURIUM AND LISTERIA MONOCYTOGENES TO CACO-2 CELLS
    Sribuathong, Supannikar
    Saengprakai, Janpen
    Trevanich, Sudsai
    JOURNAL OF FOOD SAFETY, 2014, 34 (04) : 270 - 282
  • [24] Listeria monocytogenes and Salmonella enterica Enteritidis Biofilms Susceptibility to Different Disinfectants and Stress-Response and Virulence Gene Expression of Surviving Cells
    Rodrigues, Diana
    Cerca, Nuno
    Teixeira, Pilar
    Oliveira, Rosario
    Ceri, Howard
    Azeredo, Joana
    MICROBIAL DRUG RESISTANCE, 2011, 17 (02) : 181 - 189
  • [25] PCR detection of virulence-associated genes in Campylobacter jejuni strains with differential ability to invade Caco-2 cells and to colonize the chick gut
    Müller, J
    Schulze, F
    Müller, W
    Hänel, I
    VETERINARY MICROBIOLOGY, 2006, 113 (1-2) : 123 - 129
  • [26] Elevated iron status increases bacterial invasion and survival and alters cytokine/chemokine mRNA expression in Caco-2 human intestinal cells
    Foster, SL
    Richardson, SH
    Failla, ML
    JOURNAL OF NUTRITION, 2001, 131 (05): : 1452 - 1458
  • [27] Listeria monocytogenes Strains Underrepresented during Selective Enrichment with an ISO Method Might Dominate during Passage through Simulated Gastric Fluid and In Vitro Infection of Caco-2 Cells
    Zilelidou, Evangelia
    Karmiri, Christina-Vasiliki
    Zoumpopoulou, Georgia
    Mavrogonatou, Eleni
    Kletsas, Dimitris
    Tsakalidou, Effie
    Papadimitriou, Konstantinos
    Drosinos, Eleftherios
    Skandamis, Panagiotis
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2016, 82 (23) : 6846 - 6858
  • [28] Impact of Caffeic Acid and Quercetin on the Antioxidant Machinery Expression on Caco-2 Cells is Consistent with Changes in the Cytoplasmic Disulfide Transmission Capacity
    Hidalgo, Miltha
    Kallens, Violeta
    Tobar, Nicolas
    Porras, Omar
    FREE RADICAL BIOLOGY AND MEDICINE, 2019, 145 : S59 - S59
  • [29] The Influence of Heat-Killed Enterococcus faecium BGPAS1-3 on the Tight Junction Protein Expression and Immune Function in Differentiated Caco-2 Cells Infected With Listeria monocytogenes ATCC 19111
    Popovic, Nikola
    Djokic, Jelena
    Brdaric, Emilija
    Dinic, Miroslav
    Terzic-Vidojevic, Amarela
    Golic, Natasa
    Veljovic, Katarina
    FRONTIERS IN MICROBIOLOGY, 2019, 10
  • [30] Marked inter-strain heterogeneity in the differential expression of some key stress response and virulence-related genes between planktonic and biofilm cells in Listeria monocytogenes
    Toliopoulos, Christos
    Giaouris, Efstathios
    INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2023, 390
1234