Influenza C Virus NS1 Protein Upregulates the Splicing of Viral mRNAs

被引:18
|
作者
Muraki, Yasushi [2 ]
Furukawa, Takatoshi
Kohno, Yoshihiko [3 ]
Matsuzaki, Yoko
Takashita, Emi [4 ]
Sugawara, Kanetsu
Hongo, Seiji [1 ]
机构
[1] Yamagata Univ, Sch Med, Dept Infect Dis, Fac Med, Yamagata 9909585, Japan
[2] Kanazawa Med Univ, Sch Med, Dept Microbiol, Uchinada, Ishikawa 9200293, Japan
[3] Mitsubishi Chem Medience Corp, Drug Anal Dept, Kashima Lab, Div Toxicol Sci,Medi Chem Business Segment, Kamisu City, Ibaraki 3140255, Japan
[4] Natl Inst Infect Dis, Influenza Virus Res Ctr, Tokyo 2080011, Japan
基金
日本学术振兴会;
关键词
ION-CHANNEL ACTIVITY; CORD-LIKE STRUCTURES; MATRIX PROTEIN; INFECTED-CELLS; M(2) PROTEIN; CM2; PROTEIN; NUCLEOCYTOPLASMIC TRANSPORT; MONOCLONAL-ANTIBODIES; M1; SEGMENT;
D O I
10.1128/JVI.01627-09
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Pre-mRNAs of the influenza A virus M and NS genes are poorly spliced in virus-infected cells. By contrast, in influenza C virus-infected cells, the predominant transcript from the M gene is spliced mRNA. The present study was performed to investigate the mechanism by which influenza C virus M gene-specific mRNA (M mRNA) is readily spliced. The ratio of M1 encoded by a spliced M mRNA to CM2 encoded by an unspliced M mRNA in influenza C virus-infected cells was about 10 times larger than that in M gene-transfected cells, suggesting that a viral protein(s) other than M gene translational products facilitates viral mRNA splicing. RNase protection assays showed that the splicing of M mRNA in infected cells was much higher than that in M gene-transfected cells. The unspliced and spliced mRNAs of the influenza C virus NS gene encode two nonstructural (NS) proteins, NS1(C/NS1) and NS2(C/NS2), respectively. The introduction of premature translational termination into the NS gene, which blocked the synthesis of the C/NS1 and C/NS2 proteins, drastically reduced the splicing of NS mRNA, raising the possibility that C/NS1 or C/NS2 enhances viral mRNA splicing. The splicing of influenza C virus M mRNA was increased by coexpression of C/NS1, whereas it was reduced by coexpression of the influenza A virus NS1 protein (A/NS1). The splicing of influenza A virus M mRNA was also increased by coexpression of C/NS1, though it was inhibited by that of A/NS1. These results suggest that influenza C virus NS1, but not A/NS1, can upregulate viral mRNA splicing.
引用
收藏
页码:1957 / 1966
页数:10
相关论文
共 50 条
  • [21] The Influenza A Virus Non-structural Protein NS1 Upregulates The Expression of Collagen Triple Helix Repeat Containing 1 Protein
    Zhu, C.
    Peng, G.
    Yi, W.
    Song, H.
    Liu, F.
    Liu, X.
    SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 2016, 84 (06) : 365 - 369
  • [22] PL Domain at the Carboxyl Terminus of Influenza A Virus NS1 Protein Influences The Nuclear Localization of NS1
    Zhang Dan-Gui
    Li Wei-Zhong
    Wang Ge-Fei
    Zhang Heng
    Zeng Jun
    Chen You-Ying
    Zhang Chi
    Zeng Xiang-Xing
    Li Kang-Sheng
    PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS, 2010, 37 (09) : 975 - 982
  • [23] Mutation analysis of a recombinant NS replicon shows that influenza virus NS1 protein blocks the splicing and nucleo-cytoplasmic transport of its own viral mRNA
    Garaigorta, Urtzi
    Ortin, Juan
    NUCLEIC ACIDS RESEARCH, 2007, 35 (14) : 4573 - 4582
  • [24] MOV10 sequesters the RNP of influenza A virus in the cytoplasm and is antagonized by viral NS1 protein
    Li, Jian
    Hu, Siqi
    Xu, Fengwen
    Mei, Shan
    Liu, Xiaoman
    Yin, Lijuan
    Zhao, Fei
    Zhao, Xiaoxiao
    Sun, Hong
    Xiong, Zichen
    Zhang, Di
    Cen, Shan
    Wang, Jianwei
    Liang, Chen
    Guo, Fei
    BIOCHEMICAL JOURNAL, 2019, 476 : 467 - 481
  • [25] MOV10 sequesters the RNP of influenza A virus in the cytoplasm and is antagonized by viral NS1 protein
    Li, J.
    Hu, S.
    Xu, F.
    Mei, S.
    Liu, X.
    Yin, L.
    Zhao, F.
    Zhao, X.
    Sun, H.
    Xiong, Z.
    Zhang, D.
    Cen, S.
    Wang, J.
    Liang, C.
    Guo, F.
    EUROPEAN JOURNAL OF IMMUNOLOGY, 2019, 49 : 1074 - 1074
  • [26] Effects of influenza A virus NS1 protein on protein expression:: the NS1 protein enhances translation and is not required for shutoff of host protein synthesis
    Salvatore, M
    Basler, CF
    Parisien, JP
    Horvath, CM
    Bourmakina, S
    Zheng, HY
    Muster, T
    Palese, P
    García-Sastre, A
    JOURNAL OF VIROLOGY, 2002, 76 (03) : 1206 - 1212
  • [27] hnRNP A2/B1 interacts with influenza A viral protein NS1 and inhibits virus replication potentially through suppressing NS1 RNA/protein levels and NS1 mRNA nuclear export
    Wang, Yimeng
    Zhou, Jianhong
    Du, Yuchun
    VIROLOGY, 2014, 449 : 53 - 61
  • [28] Binding of the influenza virus NS1 protein to model genome RNAs
    Hatada, E
    Saito, S
    Okishio, N
    Fukuda, R
    JOURNAL OF GENERAL VIROLOGY, 1997, 78 : 1059 - 1063
  • [29] Influenza virus NS1 protein induces apoptosis in cultured cells
    Schultz-Cherry, S
    Dybdahl-Sissoko, N
    Neumann, G
    Kawaoka, Y
    Hinshaw, VS
    JOURNAL OF VIROLOGY, 2001, 75 (17) : 7875 - 7881
  • [30] NS1 protein of influenza A virus down-regulates apoptosis
    Zhirnov, OP
    Konakova, TE
    Wolff, T
    Klenk, HD
    JOURNAL OF VIROLOGY, 2002, 76 (04) : 1617 - 1625