Generation of oxygen gradients with arbitrary shapes in a microfluidic device

被引：86
|
作者：
Adler, Micha [1 ]
Polinkovsky, Mark [1 ]
Gutierrez, Edgar [1 ]
Groisman, Alex [1 ]
机构：
[1] Univ Calif San Diego, Dept Phys, La Jolla, CA 92093 USA
来源：
LAB ON A CHIP | 2010年 / 10卷 / 03期
基金：
美国国家科学基金会;
关键词：
CELL-CULTURE; POLY(DIMETHYLSILOXANE); ANGIOGENESIS; BACTERIA; HYPOXIA; SYSTEMS;
D O I：
10.1039/b920401f
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We present a system consisting of a microfluidic device made of gas-permeable polydimethylsiloxane (PDMS) with two layers of microchannels and a computer-controlled multi-channel gas mixer. Concentrations of oxygen in the liquid-filled flow channels of the device are imposed by flowing gas mixtures with desired oxygen concentrations through gas channels directly above the flow channels. Oxygen gradients with different linear, exponential, and non-monotonic shapes are generated in the same liquid-filled microchannel and reconfigured in real time. The system can be used to study directed migration of cells and the development of cell and tissue cultures under gradients of oxygen.
引用
收藏
页码：388 / 391
页数：4
相关论文
共 50 条
  • [31] GENERATION AND TRANSPORT OF BUBBLES IN A MICROFLUIDIC DEVICE
    Xiong, Renqiang
    Chung, Jacob N.
    [J]. IMECE 2008: HEAT TRANSFER, FLUID FLOWS, AND THERMAL SYSTEMS, VOL 10, PTS A-C, 2009, : 55 - 57
  • [32] Generation of solution and surface gradients using microfluidic systems
    Jeon, NL
    Dertinger, SKW
    Chiu, DT
    Choi, IS
    Stroock, AD
    Whitesides, GM
    [J]. LANGMUIR, 2000, 16 (22) : 8311 - 8316
  • [33] Efficient Generation of Dilution Gradients With Digital Microfluidic Biochips
    Bhattacharjee, Sukanta
    Banerjee, Ansuman
    Ho, Tsung-Yi
    Chakrabarty, Krishnendu
    Bhattacharya, Bhargab B.
    [J]. IEEE TRANSACTIONS ON COMPUTER-AIDED DESIGN OF INTEGRATED CIRCUITS AND SYSTEMS, 2019, 38 (05) : 874 - 887
  • [34] Generation of complex, static solution gradients in microfluidic channels
    Wu, HK
    Huang, B
    Zare, RN
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (13) : 4194 - 4195
  • [35] Monodispersed microfluidic droplet generation by shear focusing microfluidic device
    Tan, YC
    Cristini, V
    Lee, AP
    [J]. SENSORS AND ACTUATORS B-CHEMICAL, 2006, 114 (01): : 350 - 356
  • [36] Simple microfluidic device for studying chemotaxis in response to dual gradients
    Moussavi-Harami, S. F.
    Pezzi, H. M.
    Huttenlocher, A.
    Beebe, D. J.
    [J]. BIOMEDICAL MICRODEVICES, 2015, 17 (03) : 1 - 11
  • [37] Simple microfluidic device for studying chemotaxis in response to dual gradients
    S. F. Moussavi-Harami
    H. M. Pezzi
    A. Huttenlocher
    D. J. Beebe
    [J]. Biomedical Microdevices, 2015, 17
  • [38] Cell infection within a microfluidic device using virus gradients
    Walker, GM
    Ozers, MS
    Beebe, DJ
    [J]. SENSORS AND ACTUATORS B-CHEMICAL, 2004, 98 (2-3): : 347 - 355
  • [39] Endothelialized Biomimetic Microfluidic Oxygen Transfer Device
    Epshteyn, A. A.
    Flores, E.
    Koo, A.
    Garcia-Cardena, G.
    Borenstein, J. T.
    [J]. TISSUE ENGINEERING PART A, 2014, 20 : S84 - S84
  • [40] Development of a biomimetic microfluidic oxygen transfer device
    Gimbel, A. A.
    Flores, E.
    Koo, A.
    Garcia-Cardena, G.
    Borenstein, J. T.
    [J]. LAB ON A CHIP, 2016, 16 (17) : 3227 - 3234
12345