Flavobacterium tiangeerense sp nov., a cold-living bacterium isolated from a glacier

A novel cold-living, Gram-stain-negative, yellow-pigmented and obligately aerobic bacterium designated strain 0563(T) was isolated from the China No. 1 glacier. Phylogenetic analysis showed that strain 0563(T) was a member of the genus Flavobacterium, sharing the highest 16S rRNA gene sequence similarities with Flavobacterium limicola JCM 11473(T) (98.5%) and Flavobacterium psychrolimnae NBRC 102679(T) (98.2%). The genomic DNA G + C content was 34.8 mol%. Strain 0563(T) displayed the common phenotypic and chemotaxonomic features of the genus Flavobacterium, containing menaquinone-6 (MK-6) as the major quinone and iso-c(15:0), iso-C-15:0 3-OH, summed feature 3 (comprising C-16:1 omega 7c and/or C-16:1 omega 6c), C-15:1 omega 6c(1) iso-C-15:1 G and iso-C-16:0 3-OH as the major fatty acids. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, the novel species Flavobacterium tiangeerense sp. nov. is proposed; the type strain is 0563(T) (=CGMCC 1.6847(T) = JCM 15087(T)).