Flavobacterium laiguense sp. nov., a psychrophilic bacterium isolated from Laigu glacier on the Tibetan Plateau

One bacterial strain, denoted as LB2P30(T), was isolated from Laigu glacier located on the Tibetan Plateau, PR China. Strain LB2P30(T) was an aerobic, non-motile, rod-shaped, yellow-pigmented and Gram-stain-negative bacterium. The temperature range for growth was 4-20 degrees C (optimum, 14 degrees C). The phylogenetic analysis based on partial 16S rRNA gene sequences showed that strain LB2P30(T) belonged to the genus Flavobacterium and was most similar to Flavobacterium fluvii (98.12%) and Flavobacterium limicola (97.91 %). The average nucleotide identity values between strain LB2P30(T) and its closest relatives, F. fluvii DSM 19978(T) and F. limicola DSM 15094(T), were 76.73 % and 77.40 %, respectively. Cells of strain LB2P30(T) contained summed feature 3 (comprising C-16:1 omega 7c and/or C-16:1 omega 6c) and iso-C-15:0 as the predominant fatty acids and menaquinone-6 as the sole menaquinone. The genomic DNA G+C content was 34.74 mol%. Based on the phenotypic, chemotaxonomic, genotypic and phylogenetic characteristics, we propose strain LB2P30(T) as a novel species of the genus Flavobacterium with the nomenclature of Flavobacterium laiguense sp. nov. The type strain is LB2P30(T) (=CGMCC 1.11271(T)=NBRC 113059(T)).