Cell-surface marking of CD34(+)-restricted phenotypes of human hematopoietic progenitor cells by retrovirus-mediated gene transfer

Human CD34(+) cells lacking detectable levels of HLA-DR antigens (CD34(+) DR-) are highly enriched in hematopoietic pluripotent progenitors with long-term marrow repopulating ability, We investigated the feasibility of transducing and marking CD34(+) DR- progenitor cells from bone marrow (BM) or mobilized peripheral blood samples (MPB) of 13 patients undergoing BM transplantation with the purpose of developing a protocol for a large-scale clinical application, A new retroviral vector coding for the truncated form (Delta) of the low-affinity nerve growth factor receptor (LNGFR) was used to quantitate the level of gene transfer into CD34(+) cells and their progeny by multiparameter cytofluorimetry and immunocytochemistry. Light-density mononuclear cells as well as purified CD34(+) cells were transduced either by direct incubation with retroviral supernatants or prestimulated in vitro with various combinations of growth factors prior to transduction, Transduction efficiency, assessed as G418-resistant growth of granulocyte-macrophage colony-forming units (CFU-GM) progenitors from MPB, was 1.7-fold higher (14.9% +/- 4.5%) than those from BM (8.5% +/- 3.9%) and it was further improved (26.9% +/- 3.1%) using a purified CD34(+) population as target cells, Three-color fluorescence-activated cell sorting (FACS) analysis demonstrated the presence of transduced Delta LNGFR(+) cells within the CD34(+) DR- subpopulation. In the absence of growth factors, gene transfer into BM or MPB CD34(+) DR- cells was generally poor, but following a 72-hr prestimulation it peaked at 38% of total CD34(+) DR- bone marrow (BM) cells in the presence of the c-kit ligand (KL) and at 31% in the presence of IL-3, Furthermore, KL gave, compared to the other cytokines, the highest absolute yield of BM Delta LNGFR(+) CD34(+) DR- cells recovered after transduction (p = 0.05 compared to 24 hr), Gene transfer into in vitro primitive progenitor cells was further confirmed by expression of the Delta LNGFR marker on CD34(+) cells and CFU-GM derived from 5-week long-term culture on stroma.