Overexpression of a new Cys2/His2 zinc finger protein ZmZF1 from maize confers salt and drought tolerance in transgenic Arabidopsis

被引:22
|
作者
Huai, Junling [1 ,2 ]
Zheng, Jun [1 ]
Wang, Guoying [1 ]
机构
[1] Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
[2] China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China
关键词
Cys(2)/His(2) zinc finger; Maize; Salt and drought stress; Transgenic Arabidopsis; SEQUENCE RECOGNITION; CRYSTAL-STRUCTURE; DNA RECOGNITION; SALINITY STRESS; GENE FAMILY; EAR-MOTIF; EXPRESSION; REPRESSORS; PETUNIA; GENOME;
D O I
10.1007/s11240-009-9582-2
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A new Cys(2)/His(2) zinc finger protein gene, ZmZF1, was amplified from the full-length cDNA library of osmotically stressed maize seedlings. It encodes a putative zinc finger transcription factor with two conserved C2H2 domains. Transient expression analysis of a ZmZF1-GFP fusion protein showed that ZmZF1 was preferentially localized to the nucleus, even though it carries no putative nuclear location signal. The transcript of ZmZF1 was strongly induced by salt stress. It was also induced by ABA and drought stress. Overexpression of ZmZF1 under the control of the cauliflower mosaic virus 35S promoter enhanced the salt and drought tolerance of transgenic Arabidopsis at the young seedling stage.
引用
收藏
页码:117 / 124
页数:8
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