Crystallization and preliminary X-ray characterization of a ferritin from the hyperthermophilic archaeon and anaerobe Pyrococcus furiosus

被引:13
|
作者
Matias, PM
Tatur, J
Carrondo, MA
Hagen, WR
机构
[1] Univ Nova Lisboa, Inst Tecnol Quim & Biol, P-2784505 Oeiras, Portugal
[2] Delft Univ Technol, Dept Biotechnol, NL-2628 BC Delft, Netherlands
关键词
D O I
10.1107/S1744309105011516
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Crystals of the title protein have been produced and preliminary structural analysis has been carried out. The crystals belong to the orthorhombic space group C222(1), with unit-cell parameters a = 258.1, b = 340.1, c = 266.5 angstrom. The protein forms a 24-mer of 20 kDa subunits, which assemble with 432 non-crystallographic symmetry. A total of 36 monomers are found in the asymmetric unit, corresponding to one and a half 24-mers.
引用
收藏
页码:503 / 506
页数:4
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