Studies on a novel minor-groove targeting artificial nuclease: Synthesis and DNA binding behavior

Nucleases play an important role in molecular biology, for example, in DNA sequencing. Synthetic polyamide conjugates can be considered as a novel tool for the selective inhibition of gene expressions and also as potential drugs in anticancer or antiviral chemotherapy. In this article, the synthesis of a novel minor-groove targeting artificial nuclease, an oligopyrrol-containing compound, has been reported. It was found that this novel compound can bind DNA in AT-rich minor groove with high affinity and site specificity. DNA binding behavior was determined by using UV-Vis and CD. It is indicated that compound 6 can enhance the T of DNA from 80.4 degrees C to 84.4 degrees C and that it possesses a high binding constant value(Kb = 3.05 x 10(4) L/mol)