Isolation and characterization of 11 microsatellite markers for the large yellow croaker, Pseudosciaena crocea

被引:6
|
作者
Chang, Y. M. [1 ]
Ding, L. [2 ]
Wang, W. W. [3 ]
He, J. G. [4 ]
Liang, L. Q. [1 ]
Lei, Q. Q. [5 ]
机构
[1] Chinese Acad Fisheries Sci, Heilongjiang River Fisheries Res Inst, Harbin 150070, Peoples R China
[2] Dalian Fisheries Univ, Life Sci Technol Inst, Dalian 116023, Peoples R China
[3] Harbin Inst Technol, Dept Life Sci & Engn, Harbin 150001, Peoples R China
[4] Zhongshan Univ, Coll Life Sci, Guangzhou 510275, Guangdong, Peoples R China
[5] Harbin Univ Sci & Technol, Coll Elect & Elect Engn, Harbin 150080, Peoples R China
基金
国家高技术研究发展计划(863计划);
关键词
Pseudosciaena crocea; Microsatellite markers; Enriched library; Genetic diversity;
D O I
10.1007/s10592-008-9708-9
中图分类号
X176 [生物多样性保护];
学科分类号
090705 ;
摘要
A new set of 11 microsatellite markers was isolated and characterized in the large yellow croaker (Pseudosciaena crocea Richardson) from a CA-enriched genomic library. Of 35 microsatellite markers screened, 11 microsatellite markers are highly polymorphic in one hatchery stock which contained 58 individuals. The high genetic variability was represented mainly by the number of alleles, which ranged from 7 to 16, and the observed heterozygosity, which ranged from 0.43 to 0.79. These newly isolated markers increase the available molecular resources that can be used to analyze genetic diversity and population structure of large yellow croaker.
引用
收藏
页码:1405 / 1408
页数:4
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