Isolation and characterization of novel polymorphic microsatellite loci in large yellow croaker (Larimichthys crocea)

被引：0

作者：

Wang, X. Q. ^{[1
]}

Zeng, D. ^{[1
]}

Ma, X. ^{[1
,2
]}

Xiong, G. ^{[1
]}

Wang, Z. Y. ^{[1
,3
]}

Sakamoto, T. ^{[4
]}

机构：

[1] Hunan Agr Univ, Coll Anim Sci & Technol, Collaborat Innovat Ctr Efficient & Hlth Prod Fish, Changsha, Hunan, Peoples R China

[2] Henan Normal Univ, Coll Fisheries, Xinxiang, Henan, Peoples R China

[3] Jimei Univ, Key Lab Hlth Mariculture East China Sea, Minist Agr, Coll Fisheries, Xiamen, Fujian, Peoples R China

[4] Tokyo Univ Marine Sci & Technol, Dept Marine Biosci, Tokyo, Japan

来源：

GENETICS AND MOLECULAR RESEARCH | 2015年 / 14卷 / 03期

基金：

美国国家科学基金会;

关键词：

Large yellow croaker; Microsatellites; Polymorphism; Larimichthys crocea; MARKERS;

D O I：

10.4238/2015.August.14.7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The large yellow croaker (Larimichthys crocea) is one of the largest marine net-cage cultured species in the oceans around China. In the present study, we isolated and characterized 13 polymorphic microsatellite markers from genomic libraries of L. crocea. Loci were screened for 10 wild specimens from 2 sites in southeast of China. All loci were polymorphic. The number of alleles per locus ranged from 2 to 21. The expected heterozygosity ranged from 0.233 to 0.838 and observed heterozygosity ranged from 0.527 to 0.935. Eleven loci were highly informative (polymorphic information content >0.5). Significant deviation from Hardy-Weinberg equilibrium was observed at 3 loci after Bonferroni's correction. The microsatellite loci may be valuable tools for studying the genetic diversity and genetic structure for conservation planning of the fish.

引用

页码：9437 / 9440

页数：4

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