Studying the neurovascular unit: an improved blood-brain barrier model

被引：17
|
作者：
Zehendner, Christoph M. [1 ]
Luhmann, Heiko J. [1 ]
Kuhlmann, Christoph R. W. [1 ]
机构：
[1] Johannes Gutenberg Univ Mainz, Univ Med Ctr, Inst Physiol & Pathophysiol, D-55128 Mainz, Germany
来源：
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM | 2009年 / 29卷 / 12期
关键词：
blood-brain barrier; cerebral ischemia; cortical organotypic slice culture; endothelial cells; neurovascular unit; ZO-1; TIGHT JUNCTION PERMEABILITY; DISRUPTION;
D O I：
10.1038/jcbfm.2009.103
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
The blood-brain barrier (BBB) closely interacts with the neuronal parenchyma in vivo. To replicate this interdependence in vitro, we established a murine coculture model composed of brain endothelial cell (BEC) monolayers with cortical organotypic slice cultures. The morphology of cell types, expression of tight junctions, formation of reactive oxygen species, caspase-3 activity in BECs, and alterations of electrical resistance under physiologic and pathophysiological conditions were investigated. This new BBB model allows the application of techniques such as laser scanning confocal microscopy, immunohistochemistry, fluorescent live cell imaging, and electrical cell substrate impedance sensing in real time for studying the dynamics of BBB function under defined conditions. Journal of Cerebral Blood Flow & Metabolism (2009) 29, 1879-1884; doi:10.1038/jcbfm.2009.103; published online 29 July 2009
引用
收藏
页码：1879 / 1884
页数：6
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