The ARS309 chromosomal replicator of Saccharomyces cerevisiae depends on an exceptional ARS consensus sequence

被引：80
|
作者：
Theis, JF [1 ]
Newlon, CS [1 ]
机构：
[1] UNIV MED & DENT NEW JERSEY, NEW JERSEY MED SCH, DEPT MICROBIOL & MOL GENET, NEWARK, NJ 07103 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1997年 / 94卷 / 20期
关键词：
D O I：
10.1073/pnas.94.20.10786
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Autonomously replicating sequence (ARS) elements, which function as the cis-acting chromosomal replica tors in the yeast Saccharomyces cerevisiae, depend upon an essential copy of the Il-bp ARS consensus sequence (ACS) for activity, Analysis of the chromosome III replicator ARS309 unexpectedly revealed that its essential ACS differs from the canonical ACS at two positions, One of the changes observed in ARS309 inactivates other ARS elements, This atypical ACS binds the origin recognition complex efficiently and is required for chromosomal replication origin activity, Comparison of the essential ACS of ARS309 with the essential regions of other ARS elements revealed an expanded 17-bp conserved sequence that efficiently predicts the essential core of ARS elements.
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页码：10786 / 10791
页数：6
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