Expression of bifunctional genes encoding xylanase and β(1,3-1,4)-glucanase in Gram-positive bacteria

被引：0

作者：

Ekinci, MS ^{[1
]}

Flint, HJ

机构：

[1] Kahramanmaras Sutcu Imam Univ, Fac Agr, Dept Anim Sci, Kahramanmaras, Turkey

[2] Rowett Res Inst, Aberdeen AB21 9SB, Scotland

来源：

TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES | 2001年 / 25卷 / 05期

关键词：

gene expression; Gram-positive bacteria; enzyme;

D O I：

暂无

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The xylanase and beta (1,3-1,4)-Glucanase activities encoding gene from the cellulolytic rumen anaerobe bacterium was cloned into Escherichia/Streptococcus shuttle vector for introduction into Gram-positive bacteria, including the rumen facultative anaerobe bacterium Streptococcus bovis. Activities due to the cloned gene decreased in the stationary phase in batch cultures of S. bovis, reflecting the sensitivity of the cloned enzymes to inactivation in the presence of accumulated lactic acid. Cloned gene activity was detected in the culture supernatant, indicating recognition of the cloned gene signal peptide by Gram-positive bacteria.

引用

页码：771 / 775

页数：5

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