Development of ultra-high-performance supercritical fluid chromatography-mass spectrometry assays to analyze potential biomarkers in sweat

被引:4
|
作者
Herniman, Julie M. [1 ]
Worsley, Peter R. [2 ]
Greenhill, Rachel [1 ]
Bader, Dan L. [2 ]
Langley, G. John [1 ]
机构
[1] Univ Southampton, Fac Engn & Phys Sci, Sch Chem, Southampton SO17 1BJ, Hants, England
[2] Univ Southampton, Fac Environm & Hlth Sci, Sch Hlth Sci, Southampton, Hants, England
基金
英国工程与自然科学研究理事会;
关键词
acids; mass spectrometry; purines; sweat; ultra-high-performance supercritical fluid chromatography; LACTATE-PYRUVATE RATIO; LIQUID-CHROMATOGRAPHY; PURINE;
D O I
10.1002/jssc.202100261
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Liquid chromatography-mass spectrometry methods were required to afford the rapid separation and detection of purines and small organic acids. These compounds are found in sweat and sebum and are potential biomarkers for the early detection of pressures sores. Two ultra-high-performance supercritical fluid chromatography-mass spectrometry assays have been successfully developed for both classes of compounds. Separation for purines was achieved using a gradient of supercritical carbon dioxide and methanol with a 1-aminoanthracene sub 2 mu m particle size column followed by positive ion electrospray ionization. Separation for organic acids was achieved using a gradient of supercritical carbon dioxide and methanol (50 mM ammonium acetate 2% water) with a Diol sub 2 mu m particle size column followed by negative ion electrospray ionization. Calibration curves were created in the absence of internal standards and R-2 values > 0.96 were achieved using single ion monitoring methods for the protonated purines and the deprotonated acids. The two new assays afford rapid analytical methods for the separation and detection of potential biomarkers in human sweat leading to the early detection and prevention of pressure sores.
引用
收藏
页码:542 / 550
页数:10
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