First report and new molecular and morphological characterizations of root-knot nematode, Meloidogyne java']javanica, infecting ginger and long coriander in Vietnam

被引：2

作者：

Ke Long Phan ^{[1
,3
]}

Thi Mai Linh Le ^{[2
,3
]}

Huu Tien Nguyen ^{[2
,3
,4
]}

Thi Duyen Nguyen ^{[2
,3
]}

Quang Phap Trinh ^{[2
,3
]}

机构：

[1] Vietnam Acad Sci & Technol, Vietnam Natl Museum Nat, 18 Hoang Quoc Viet, Hanoi 100000, Vietnam

[2] Vietnam Acad Sci & Technol, Inst Ecol & Biol Resources, 18 Hoang Quoc Viet, Hanoi 100000, Vietnam

[3] Grad Univ Sci & Technol, Vietnam Acad Sci & Technol, Hanoi 100000, Vietnam

[4] Univ Ghent, Nematol Res Unit, Dept Biol, KL Ledeganckstr 35, B-9000 Ghent, Belgium

来源：

JOURNAL OF NEMATOLOGY | 2021年 / 53卷

关键词：

28S rRNA; ITS; mtDNA; DNA barcode; Multiplex-PCR; Taxonomy; Plant-parasitic nematodes; N; SP;

D O I：

10.21307/jofnem-2021-011

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Ginger (Zingiber officinale Roscoe) and long coriander (Eryngium foetidum L.) are commonly grown and used as important spices and medicinal plants in Vietnam. Our study recovered for the first time one of the most damaging tropical root-knot nematodes, Meloidogyne javanica, associated with these plants in the Western Highlands of Vietnam. In this study, M. javanica was characterized based on morphology and molecular characterization of D2-D3 fragment of 28S rRNA, ITS, and Nad5 mtDNA regions. The identification of this species was done based on a combination of morphology, multiplex-PCR with specific primer, network haplotype analysis, and PPNID program.

引用

页数：8

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