A technique for monitoring hydrogen peroxide concentration off-line and on-line

被引:14
|
作者
Guwy, AJ [1 ]
Hawkes, FR
Martin, SR
Hawkes, DL
Cunnah, P
机构
[1] Univ Glamorgan, Sch Appl Sci, Pontypridd CF37 1DL, M Glam, Wales
[2] Univ Glamorgan, Sch Design & Adv Technol, Pontypridd CF37 1DL, M Glam, Wales
[3] Biocatalysts Ltd, Treforest CF37 5UD, M Glam, Wales
基金
英国生物技术与生命科学研究理事会;
关键词
hydrogen peroxide; catalase; on-line monitoring;
D O I
10.1016/S0043-1354(99)00404-2
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
A simple technique using a low flow gas meter is described for the off-line and on-line measurement of hydrogen peroxide. The volume of oxygen produced from the reaction of the enzyme catalase with hydrogen peroxide was followed. A linear correlation was demonstrated between total O-2 evolution and H2O2 concentration in the range 0.25-1.5% for on-line assays and 25-500 mg/l and 0.38-3% for the off-line method. A response time of 17 min was obtained for the off-line for hydrogen peroxide concentrations of 25-500 mg/l. The response time of an on-line version of the method was approximately 5 min for hydrogen peroxide concentrations of 0.25-1.5%. The technique was not significantly temperature sensitive in the range 25-45 degrees C. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:2191 / 2198
页数:8
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