Optimization of culture conditions of the thraustochytrid Aurantiochytrium sp. strain 18W-13a for squalene production

被引：71
|
作者：
Nakazawa, Atsushi [1 ]
Matsuura, Hiroshi [1 ]
Kose, Ryoji [1 ]
Kato, Syou [1 ]
Honda, Daiske [2 ]
Inouye, Isao [1 ]
Kaya, Kunimitsu [1 ]
Watanabe, Makoto M. [1 ]
机构：
[1] Univ Tsukuba, Grad Sch Life & Environm Sci, Tsukuba, Ibaraki 3058572, Japan
[2] Konan Univ, Dept Biol, Fac Sci & Engn, Higashinada Ku, Kobe, Hyogo 6588501, Japan
来源：
BIORESOURCE TECHNOLOGY | 2012年 / 109卷
基金：
日本科学技术振兴机构;
关键词：
Aurantiochytrium; Biomass; Hydrocarbon; Squalene; Thraustochytrid; GENE PHYLOGENY THRAUSTOCHYTRIACEAE; DOCOSAHEXAENOIC ACID PRODUCTION; TAXONOMIC REARRANGEMENT; ENHANCED PRODUCTION; SCHIZOCHYTRIUM; TEMPERATURE; EMENDATION; MORPHOLOGY; ERECTION; GROWTH;
D O I：
10.1016/j.biortech.2011.09.127
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
Optimum conditions of temperature, salinity and glucose concentration were investigated for squalene production of the strain of Aurantiochytrium sp. 18W-13a, with a high content of squalene. Squalene production by this strain was optimum at 25 degrees C, 25-50% seawater concentration and 2-6% glucose concentration. When this strain was grown in the optimum condition, the squalene content and production of approximately 171 mg/g dry weight and 0.9 g/L. were much higher than that previously reported in thraustochytrids, plants and yeasts, respectively. Therefore, 18W-13a could be used as an alternative source of commercial squalene. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码：287 / 291
页数：5
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