Thraustochytrid Aurantiochytrium sp 18W-13a Accummulates High Amounts of Squalene

被引:109
|
作者
Kaya, Kunimitsu [1 ]
Nakazawa, Atsushi [1 ]
Matsuura, Hiroshi [1 ]
Honda, Daisuke [2 ]
Inouye, Isao [1 ]
Watanabe, Makoto M. [1 ]
机构
[1] Univ Tsukuba, Grad Sch Life & Environm Sci, Tsukuba, Ibaraki 3058572, Japan
[2] Konan Univ, Fac Sci & Engn, Dept Biol, Higashinada Ku, Kobe, Hyogo 6588501, Japan
基金
日本科学技术振兴机构;
关键词
squalene; thraustochytrid; Aurantiochytrium; OLIVE OIL; ENHANCED PRODUCTION; OPTIMIZATION; EXTRACTION; CANCER; OXYGEN; MICE;
D O I
10.1271/bbb.110430
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Here we report on the 18W-13a strain of Aurantiochytrium sp., which accumulates very high amounts of squalene. The squalene contents and production at 4d of culture were 198 mg/g and 1.29 +/- 0.13 g/L, respectively, exceptionally high values compared to previous reports.
引用
收藏
页码:2246 / 2248
页数:3
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共 15 条
  • [1] Optimization of culture conditions of the thraustochytrid Aurantiochytrium sp. strain 18W-13a for squalene production
    Nakazawa, Atsushi
    Matsuura, Hiroshi
    Kose, Ryoji
    Kato, Syou
    Honda, Daiske
    Inouye, Isao
    Kaya, Kunimitsu
    Watanabe, Makoto M.
    [J]. BIORESOURCE TECHNOLOGY, 2012, 109 : 287 - 291
  • [2] Identification of extracellular proteins from Aurantiochytrium sp 18W-13a
    Juntila, Darryl Joy
    Yoneda, Kohei
    Suzuki, Iwane
    [J]. JOURNAL OF APPLIED PHYCOLOGY, 2018, 30 (01) : 63 - 69
  • [3] Identification of extracellular proteins from Aurantiochytrium sp. 18W-13a
    Darryl Joy Juntila
    Kohei Yoneda
    Iwane Suzuki
    [J]. Journal of Applied Phycology, 2018, 30 : 63 - 69
  • [4] Genetic modification of the thraustochytrid Aurantiochytrium sp. 18W-13a for cellobiose utilization by secretory expression of β-glucosidase from Aspergillus aculeatus
    Juntila, Darryl Joy
    Yoneda, Kohei
    Suzuki, Iwane
    [J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, 2019, 40
  • [5] Enhancement of Squalene Production by Constitutive Expression of the 3-Hydroxy-3-Methylglutaryl-CoA Reductase in Aurantiochytrium sp. 18W-13a
    Tianjing Yang
    Darryl Joy Juntila
    Naomichi Fujihara
    Takashi Inada
    Kohei Yoneda
    Iwane Suzuki
    [J]. Marine Biotechnology, 2022, 24 : 733 - 743
  • [6] Enhancement of Squalene Production by Constitutive Expression of the 3-Hydroxy-3-Methylglutaryl-CoA Reductase in Aurantiochytrium sp. 18W-13a
    Yang, Tianjing
    Juntila, Darryl Joy
    Fujihara, Naomichi
    Inada, Takashi
    Yoneda, Kohei
    Suzuki, Iwane
    [J]. MARINE BIOTECHNOLOGY, 2022, 24 (04) : 733 - 743
  • [7] Genetic Modification of Aurantiochytrium sp. 18W-13a for Enhancement of Proteolytic Activity by Heterologous Expression of Extracellular Proteases
    Yoneda, Kohei
    Man, Chun Hung
    Maeda, Yoshiaki
    Suzuki, Iwane
    [J]. MARINE BIOTECHNOLOGY, 2024,
  • [8] Characterization of a Squalene Synthase from the Thraustochytrid Microalga Aurantiochytrium sp KRS101
    Hong, Won-Kyung
    Heo, Sun-Yeon
    Park, Hye-Mi
    Kim, Chul Ho
    Sohn, Jung-Hoon
    Kondo, Akihiko
    Seo, Jeong-Woo
    [J]. JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2013, 23 (06) : 759 - 765
  • [9] Optimization of nitrogen source for enhanced production of squalene from thraustochytrid Aurantiochytrium sp.
    Chen, Guanqun
    Fan, King-Wai
    Lu, Fu-Ping
    Li, Qian
    Aki, Tsunehiro
    Chen, Feng
    Jiang, Yue
    [J]. NEW BIOTECHNOLOGY, 2010, 27 (04) : 382 - 389
  • [10] Investigation of the Wet Extraction of Hydrocarbons from a Heterotrophic Microalga, Aurantiochytrium sp. 18W-13a, by Cell Disruption Using a High-pressure Homogenizer
    Fukuda, Yuka
    Tsutsumi, Shun
    Saito, Yasuhiro
    Matsushita, Yohsuke
    Aoki, Hideyuki
    Fukuda, Shinya
    Suzuki, Iwane
    Tani, Narumi
    [J]. JOURNAL OF THE JAPAN INSTITUTE OF ENERGY, 2018, 97 (12) : 353 - 361