Transcriptional pattern of TGF-β1 inhibitory effect on mouse C2C12 myoblasts differentiation

被引：9
|
作者：
Wicik, Z. [1 ]
Sadkowski, T. [1 ]
Jank, M. [1 ]
Motyl, T. [1 ]
机构：
[1] Warsaw Univ Life Sci SGGW, Fac Vet Med, Dept Physiol Sci, PL-02776 Warsaw, Poland
来源：
POLISH JOURNAL OF VETERINARY SCIENCES | 2010年 / 13卷 / 04期
关键词：
C2C12; myoblasts; differentiation; DNA microarrays; myogenesis; TGF-beta; 1; SKELETAL-MUSCLE; GENE-EXPRESSION; BETA; MYOGENESIS; WNT; CELLS; PATHWAYS; REPAIR; BULLS;
D O I：
10.2478/v10181-010-0008-1
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
The aim of the present study was to define the effect of TGF-beta 1 on C2C12 myoblasts myogenesis. TGF-beta 1 together with its receptor is a negative auto-paracrine regulator of myogenesis, which influences the proliferation, differentiation, and functions of muscle cells. TGF-beta 1 exerts highly significant inhibitory effect on differentiation of C2C12 mouse myoblasts manifested by the impairment of cell fusion and very low expression of myosin heavy chain. The study of differentiating C2C12 mouse myoblasts treated with TGF-beta 1 revealed 502 genes (436 down-regulated and 66 up-regulated) with statistically different expression. TGF-beta 1-regulated genes were identified to be involved in 29 biological processes, 29 molecular functions groups and 59 pathways. The strongest inhibiting effect of TGF-beta 1 was observed in the cadherin and Wnt pathways. The key-genes that could play the role of TGF-beta 1 targets during myoblasts differentiation was identified such as: Max, Creb1, Ccna2, Box, MdfI, Tef Tabg1, Cxcl5, Rho, Calca and Lgals4.
引用
收藏
页码：629 / 638
页数：10
相关论文
共 50 条
  • [41] Cell Organelle Positioning of Micropatterned Single C2C12 Mouse Myoblasts
    Guirguis, Mark
    Rimkunas, Katelyn
    Raymond, Michael
    Wan, Leo Q.
    2013 39TH ANNUAL NORTHEAST BIOENGINEERING CONFERENCE (NEBEC 2013), 2013, : 315 - 316
  • [42] Subnuclear localization and differentiation-dependent increased expression of DGK-ζ in C2C12 mouse myoblasts
    Evangelisti, Camilla
    Riccio, Massimo
    Faenza, Irene
    Zini, Nicoletta
    Hozumi, Yasukazu
    Goto, Kaoru
    Cocco, L.
    Martelli, Alberto M.
    JOURNAL OF CELLULAR PHYSIOLOGY, 2006, 209 (02) : 370 - 378
  • [43] MYOC Promotes the Differentiation of C2C12 Cells by Regulation of the TGF-β Signaling Pathways via CAV1
    Zhang, Yuhan
    Li, Shuang
    Wen, Xin
    Tong, Huili
    Li, Shufeng
    Yan, Yunqin
    BIOLOGY-BASEL, 2021, 10 (07):
  • [44] Regulation of the mouse Id2 promoter in C2C12 myoblasts.
    Gaspard, S
    Vu, S
    Liu, J
    Fan, F
    Etezadkhah, A
    Sharp, SB
    FASEB JOURNAL, 1999, 13 (07): : A1450 - A1450
  • [45] EFFECT OF LOW LEVEL LASER THERAPY IN THE PROLIFERATION OF MYOBLASTS C2C12 DURING DIFFERENTIATION PROCESS
    Mesquita-Ferrari, Raquel
    Fernandes, Kristianne
    Artilheiro, Paola
    Barbosa, Jean
    Bussadori, Sandra
    Franca, Cristiane
    Mikaele, Tavares
    LASERS IN SURGERY AND MEDICINE, 2012, 44 : 52 - 52
  • [46] Transcriptional upregulation centra of HO-1 by EGB via the MAPKs/Nrf2 pathway in mouse C2C12 myoblasts
    Wang, Jianfeng
    Zhang, Li
    Zhang, Ying
    Luo, Meiling
    Wu, Qiong
    Yu, Lijun
    Chu, Haiying
    TOXICOLOGY IN VITRO, 2015, 29 (02) : 380 - 388
  • [47] Effect of extracellular high phosphate on myogenesis of C2C12 myoblasts
    Tomatsu, Eisuke
    Inagaki, Hidehito
    Kawakami, Tsukasa
    Asada, Yohei
    Nakayama, Shogo
    Hiratsuka, Izumi
    Yoshino, Yasumasa
    Sekiguchi-Ueda, Sahoko
    Shibata, Megumi
    Takayanagi, Takeshi
    Sugimura, Yoshihisa
    Kurahashi, Hiroki
    Suzuki, Atsushi
    JOURNAL OF BONE AND MINERAL RESEARCH, 2018, 33 : 241 - 241
  • [48] Mirk/Dyrk1B mediates survival during the differentiation of C2C12 myoblasts
    Mercer, SE
    Ewton, DZ
    Deng, XB
    Lim, S
    Mazur, TR
    Friedman, E
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (27) : 25788 - 25801
  • [49] The effects of MFH-1 on BMP-2-induced osteoblastic differentiation of C2C12 myoblasts
    Fu, Y
    Deng, WG
    Yang, XL
    PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS, 2002, 29 (02) : 228 - 232
  • [50] TNF-α regulates early differentiation of C2C12 myoblasts in an autocrine fashion
    Li, YP
    Schwartz, RJ
    FASEB JOURNAL, 2001, 15 (06): : 1413 - +
12345