Home-made capillary array electrophoresis for high-throughput amino acid analysis

被引：16
|
作者：
Liu, Kaiying [1 ,2 ]
Wang, Hui [1 ]
Bai, Jiling [1 ]
Wang, Li [1 ]
机构：
[1] Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Dalian 116023, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
来源：
ANALYTICA CHIMICA ACTA | 2008年 / 622卷 / 1-2期
关键词：
capillary array electrophoresis; confocal fluorescence scanner; chiral separation; 532 nm laser;
D O I：
10.1016/j.aca.2008.05.062
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A capillary array electrophoresis (CAE) with confocal rotary scanner for high-throughput carboxytetramethy1rhodamine succinimidyl ester (TAMRA)-labeled amino acid (AA) analysis is presented. Performance of the CAE setup was evaluated with AA samples. Up to 128 capillaries could be detected in parallel. For the first time, the device was applied to separate the enantiomers of isoleucine, cysteine, and glutamic acid with cyclodextrinmodified electrolytes by capillary zone electrophoresis. Baseline separation of seven AAs is also demonstrated using micellar electrokinetic chromatography method. (c) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码：169 / 174
页数：6
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