Identification of type 4 fimbriae in Actinobacillus pleuropneumoniae

被引:5
|
作者
Zhang, YM [1 ]
Tennent, JM [1 ]
Ingham, A [1 ]
Beddome, G [1 ]
Prideaux, C [1 ]
Michalski, WP [1 ]
机构
[1] CSIRO, Australian Anim Hlth Lab, Geelong, Vic 3220, Australia
关键词
type; 4; fimbria; fimbrial protein; protein purification and sequencing; Actinobacillus pleuropneumoniae;
D O I
10.1016/S0378-1097(00)00244-5
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Type 4 fimbriae have been identified on the cell surface of Actinobacillus pleuropneumoniae by electron microscopy and N-terminal sequencing analysis. A. pleuropneumoniae type 4 fimbrial subunit protein, purified from cell cultures and from outer membrane preparations, reacted with polyclonal antibody raised against type 4 fimbriae of Moraxella bovis on Western blots. N-terminal sequence analysis of the purified 17 kDa type 4 fimbrial subunit protein, named ApfA, revealed the first 12 amino acids to be identical to those of other type 4 fimbrial subunit proteins. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:15 / 18
页数:4
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