type;
4;
fimbria;
fimbrial protein;
protein purification and sequencing;
Actinobacillus pleuropneumoniae;
D O I:
10.1016/S0378-1097(00)00244-5
中图分类号:
Q93 [微生物学];
学科分类号:
071005 ;
100705 ;
摘要:
Type 4 fimbriae have been identified on the cell surface of Actinobacillus pleuropneumoniae by electron microscopy and N-terminal sequencing analysis. A. pleuropneumoniae type 4 fimbrial subunit protein, purified from cell cultures and from outer membrane preparations, reacted with polyclonal antibody raised against type 4 fimbriae of Moraxella bovis on Western blots. N-terminal sequence analysis of the purified 17 kDa type 4 fimbrial subunit protein, named ApfA, revealed the first 12 amino acids to be identical to those of other type 4 fimbrial subunit proteins. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
机构:
Univ Vet Med, Dept Microbiol & Infect Dis, POB 22, H-1581 Budapest, HungaryUniv Vet Med, Dept Microbiol & Infect Dis, POB 22, H-1581 Budapest, Hungary
Sarkozi, Rita
Makrai, Laszlo
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机构:
Univ Vet Med, Dept Microbiol & Infect Dis, POB 22, H-1581 Budapest, HungaryUniv Vet Med, Dept Microbiol & Infect Dis, POB 22, H-1581 Budapest, Hungary
Makrai, Laszlo
Fodor, Laszlo
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机构:
Univ Vet Med, Dept Microbiol & Infect Dis, POB 22, H-1581 Budapest, HungaryUniv Vet Med, Dept Microbiol & Infect Dis, POB 22, H-1581 Budapest, Hungary
机构:
Ctr Invest & Estudios Avanzados, IPN, Dept Biol Celular, Mexico City 07000, DF, Mexico
Inst Tecnol Tlajomulco, Ctr Invest, Instrumental Anal Lab, Tlajomulco De Zuniga 45640, Jal, MexicoCtr Invest & Estudios Avanzados, IPN, Dept Biol Celular, Mexico City 07000, DF, Mexico
Serrano-Rubio, Luis E.
Tenorio-Gutierrez, Victor
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机构:
SAGARPA, Inst Nacl Invest Forestales Agr & Pecuarias, Ctr Nacl Microbiol, Mexico City 05110, DF, MexicoCtr Invest & Estudios Avanzados, IPN, Dept Biol Celular, Mexico City 07000, DF, Mexico
Tenorio-Gutierrez, Victor
Suarez-Guemes, Francisco
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机构:
Univ Nacl Autonoma Mexico, Fac Med Vet & Zootecnia, Mexico City 00900, DF, MexicoCtr Invest & Estudios Avanzados, IPN, Dept Biol Celular, Mexico City 07000, DF, Mexico
Suarez-Guemes, Francisco
Reyes-Cortes, Ruth
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机构:
Ctr Invest & Estudios Avanzados, IPN, Dept Biol Celular, Mexico City 07000, DF, MexicoCtr Invest & Estudios Avanzados, IPN, Dept Biol Celular, Mexico City 07000, DF, Mexico
Reyes-Cortes, Ruth
Rodriguez-Mendiola, Martha
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机构:
Inst Tecnol Tlajomulco, Ctr Invest, Instrumental Anal Lab, Tlajomulco De Zuniga 45640, Jal, MexicoCtr Invest & Estudios Avanzados, IPN, Dept Biol Celular, Mexico City 07000, DF, Mexico
Rodriguez-Mendiola, Martha
Arias-Castro, Carlos
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机构:
Inst Tecnol Tlajomulco, Ctr Invest, Instrumental Anal Lab, Tlajomulco De Zuniga 45640, Jal, MexicoCtr Invest & Estudios Avanzados, IPN, Dept Biol Celular, Mexico City 07000, DF, Mexico
Arias-Castro, Carlos
Godinez-Vargas, Delfino
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机构:
Ctr Invest & Estudios Avanzados, IPN, Dept Biol Celular, Mexico City 07000, DF, MexicoCtr Invest & Estudios Avanzados, IPN, Dept Biol Celular, Mexico City 07000, DF, Mexico
Godinez-Vargas, Delfino
de la Garza, Mireya
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机构:
Ctr Invest & Estudios Avanzados, IPN, Dept Biol Celular, Mexico City 07000, DF, MexicoCtr Invest & Estudios Avanzados, IPN, Dept Biol Celular, Mexico City 07000, DF, Mexico
机构:Huazhong Agricultural University,Division of Animal Infectious Diseases in State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine
Xiabing Chen
Zhuofei Xu
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机构:Huazhong Agricultural University,Division of Animal Infectious Diseases in State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine
Zhuofei Xu
Lu Li
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机构:Huazhong Agricultural University,Division of Animal Infectious Diseases in State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine
Lu Li
Huanchun Chen
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机构:Huazhong Agricultural University,Division of Animal Infectious Diseases in State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine
Huanchun Chen
Rui Zhou
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机构:Huazhong Agricultural University,Division of Animal Infectious Diseases in State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine