Multicolor 3D Super-resolution Imaging by Quantum Dot Stochastic Optical Reconstruction Microscopy

被引：74

作者：

Xu, Jianquan ^{[1
]}

Tehrani, Kayvan F. ^{[1
]}

Kner, Peter ^{[1
]}

机构：

[1] Univ Georgia, Coll Engn, Athens, GA 30602 USA

来源：

ACS NANO | 2015年 / 9卷 / 03期

基金：

美国国家科学基金会;

关键词：

fluorescence microscopy; quantum dots; super-resolution microscopy; stochastic optical reconstruction microscopy; SUBDIFFRACTION-RESOLUTION; CELLS; MITOCHONDRIA; BLINKING; LIMIT; LUMINESCENCE; NANOSCOPY; BREAKING;

D O I：

10.1021/nn506952g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We demonstrate multicolor three-dimensional super-resolution imaging with quantum dots (QSTORM). By combining quantum dot asynchronous spectral blueing with stochastic optical reconstruction microscopy and adaptive optics, we achieve three-dimensional imaging with 24 nm lateral and 37 nm axial resolution. By pairing two short-pass filters with two appropriate quantum dots, we are able to image single blueing quantum dots on two channels simultaneously, enabling multicolor imaging with high photon counts.

引用

页码：2917 / 2925

页数：9

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