Triplex real-time PCR assay for the detection of Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae directly from clinical specimens without extraction of DNA

被引：13
|
作者：
Ouattara, Mahamoudou [1 ]
Whaley, Melissa J. [1 ]
Jenkins, Laurel T. [1 ]
Schwartz, Stephanie B. [1 ]
Traore, Rasmata Ouedraogo [2 ]
Diana, Seydou [3 ]
Collard, Jean-Marc [4 ,5 ]
Sacchi, Claudio T. [6 ]
Wang, Xin [1 ]
机构：
[1] Ctr Dis Control & Prevent, Div Bacterial Dis, Atlanta, GA 30333 USA
[2] Ctr Hosp Univ Pediat Charles de Gaulle, Ouagadougou, Burkina Faso
[3] Inst Natl Rech Sante Publ, Bamako, Mali
[4] Pasteur Inst Madagascar, Antananarivo, Madagascar
[5] Ctr Rech Med & Sanitaire, Niamey, Niger
[6] Adolfo Lutz Inst, Sao Paulo, Brazil
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2019年 / 93卷 / 03期
关键词：
Meningitis; Triplex; Real-time PCR; Detection; Cerebrospinal; Bacteria; PNEUMOCOCCAL MENINGITIS; BACTERIAL PATHOGENS; AGREEMENT; ADULTS;
D O I：
10.1016/j.diagmicrobio.2018.10.008
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
This study presents a triplex real-time PCR assay that allows for the direct detection of Streptococcus pneumoniae, Neisseria meningitidis, and Haemophilus influenzae in one reaction without DNA extraction, with similar sensitivity and specificity to singleplex assays. This approach saves time, specimen volume and reagents while achieving a higher testing throughput.
引用
收藏
页码：188 / 190
页数：3
相关论文
共 50 条
  • [1] Real-time PCR detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae DNA in clinical specimens
    Vackova, Z.
    Lzicarova, D.
    Stock, N. K.
    Kozakova, J.
    [J]. EPIDEMIOLOGIE MIKROBIOLOGIE IMUNOLOGIE, 2015, 64 (04): : 222 - 230
  • [2] Simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in suspected cases of meningitis and septicemia using real-time PCR
    Corless, CE
    Guiver, M
    Borrow, R
    Edwards-Jones, V
    Fox, AJ
    Kaczmarski, EB
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (04) : 1553 - 1558
  • [3] Accuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosis
    Wu, Henry M.
    Cordeiro, Soraia M.
    Harcourt, Brian H.
    Carvalho, Maria da Gloria S.
    Azevedo, Jailton
    Oliveira, Tainara Q.
    Leite, Mariela C.
    Salgado, Katia
    Reis, Mitermayer G.
    Plikaytis, Brian D.
    Clark, Thomas A.
    Mayer, Leonard W.
    Ko, Albert I.
    Martin, Stacey W.
    Reis, Joice N.
    [J]. BMC INFECTIOUS DISEASES, 2013, 13
  • [4] Accuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosis
    Henry M Wu
    Soraia M Cordeiro
    Brian H Harcourt
    Maria da Gloria S Carvalho
    Jailton Azevedo
    Tainara Q Oliveira
    Mariela C Leite
    Katia Salgado
    Mitermayer G Reis
    Brian D Plikaytis
    Thomas A Clark
    Leonard W Mayer
    Albert I Ko
    Stacey W Martin
    Joice N Reis
    [J]. BMC Infectious Diseases, 13
  • [5] Diagnosis of bacterial meningitis caused by Streptococcus pneumoniae, Neisseria meningitidis, and Haemophilus influenzae using a multiplex real-time PCR technique
    Noemi Carnalla-Barajas, Maria
    Soto-Nogueron, Araceli
    Martinez-Medina, Lucila
    Elizabeth Olvera-Herrera, Maria
    Luis Mosqueda-Gomez, Juan
    Rodriguez-Cortez, Patricia
    Morfin-Otero, Rayo
    Rodriguez-Noriega, Eduardo
    Luevanos-Velazquez, Antonio
    Merlo-Palomera, Mariana
    Esparza-Ahumada, Sergio
    Marquez-Diaz, Francisco
    Mercedes Alpuche-Aranda, Celia
    Echaniz-Aviles, Gabriela
    [J]. BRAZILIAN JOURNAL OF MICROBIOLOGY, 2022, 53 (04) : 1951 - 1958
  • [6] Diagnosis of bacterial meningitis caused by Streptococcus pneumoniae, Neisseria meningitidis, and Haemophilus influenzae using a multiplex real-time PCR technique
    María Noemí Carnalla-Barajas
    Araceli Soto-Noguerón
    Lucila Martínez-Medina
    Maria Elizabeth Olvera-Herrera
    Juan Luis Mosqueda-Gómez
    Patricia Rodríguez-Cortez
    Rayo Morfin-Otero
    Eduardo Rodriguez-Noriega
    Antonio Luévanos-Velázquez
    Mariana Merlo-Palomera
    Sergio Esparza-Ahumada
    Francisco Márquez-Díaz
    Celia Mercedes Alpuche-Aranda
    Gabriela Echaniz-Aviles
    [J]. Brazilian Journal of Microbiology, 2022, 53 : 1951 - 1958
  • [7] Simultaneous single-tube PCR assay for the detection of Neisseria meningitidis, Haemophilus influenzae type b and Streptococcus pneumoniae
    Tzanakaki, G
    Tsopanomichalou, M
    Kesanopoulos, K
    Matzourani, R
    Sioumala, M
    Tabaki, A
    Kremastinou, J
    [J]. CLINICAL MICROBIOLOGY AND INFECTION, 2005, 11 (05) : 386 - 390
  • [8] Direct Real-Time PCR for the Detection and Serotyping of Haemophilus influenzae without DNA Extraction
    Marasini, Daya
    Whaley, Melissa J.
    Jenkins, Laurel T.
    Hu, Fang
    Jiang, Wenxin
    Topaz, Nadav
    Chen, Alex
    Schmink, Susanna
    Dolan Thomas, Jennifer
    Harcourt, Brian H.
    Marjuki, Henju
    Wang, Xin
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2022, 60 (04)
  • [9] A duplex real-time PCR for the detection of Streptococcus pneumoniae and Neisseria meningitidis in cerebrospinal fluid
    Diawara, Idrissa
    Katfy, Khalid
    Zerouali, Khalid
    Belabbes, Houria
    Elmdaghri, Naima
    [J]. JOURNAL OF INFECTION IN DEVELOPING COUNTRIES, 2016, 10 (01): : 53 - 61
  • [10] Comparison of PCR-based methods for the simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in clinical samples
    de Filippis, Ivano
    de Andrade, Claudia Ferreira
    Caldeira, Nathalia
    de Azevedo, Aline Carvalho
    de Almeida, Antonio Eugenio
    [J]. BRAZILIAN JOURNAL OF INFECTIOUS DISEASES, 2016, 20 (04): : 335 - 341
12345