Reintroduction of the 2G12 epitope in an HIV-1 clade C gp120

被引：19
|
作者：
Chen, HY [1 ]
Xu, XD [1 ]
Bishop, A [1 ]
Jones, IM [1 ]
机构：
[1] Univ Reading, Sch Anim & Microbial Sci, Reading RG6 6AJ, Berks, England
来源：
AIDS | 2005年 / 19卷 / 08期
基金：
英国医学研究理事会;
关键词：
D O I：
10.1097/01.aids.0000168980.74713.9e
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Many clade C isolates of HIV-1 do not react with monoclonal antibody (MAb) 2G12, a broad-ranging human neutralizing MAb that recognizes high mannose carbohydrate groups attached to glycoprotein gp120. We reintroduced a partial and complete 2G12 epitope into a clade C background, HIV-1(CN54), and examined the antibody reactivity of the resulting recombinant molecules. Two glycosylation sites recovered 2G12 binding completely, but some binding was evident after the reintroduction of a single glycosylation site at Asn295.
引用
收藏
页码：833 / 835
页数：3
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