Neurotransmitter release from semi-intact synaptosomes

被引：8
|
作者：
Tandon, A
Tan, PK
Bannykh, S
Banerjee, A
Balch, WE
机构：
[1] Scripps Res Inst, Dept Cell & Mol Biol, La Jolla, CA 92037 USA
[2] Univ Wisconsin, Dept Food Microbiol & Toxicol, Madison, WI 53706 USA
来源：
METHODS | 1998年 / 16卷 / 02期
关键词：
D O I：
10.1006/meth.1998.0667
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We have developed a secretion assay composed of semi-intact synaptosomes from which transmitter release is optimally evoked by micromolar Ca2+ in the presence of cytosol. Transmitter release from this preparation reconstitutes known characteristics of regulated exocytosis and is accompanied by a marked decrease in synaptic vesicles. The assay is useful in characterizing the components known to be involved in transmitter release, and should also facilitate the identification of additional factors that are important for this process. (C) 1998 Academic Press.
引用
收藏
页码：198 / 203
页数：6
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