Effect of Wounding on Gene Expression Involved in Artemisinin Biosynthesis and Artemisinin Production in Artemisia annua

被引：17
|
作者：
Liu, Donghui [1 ,3 ]
Zhang, Lida [1 ]
Li, Chengxiang [1 ]
Yang, Ke [1 ]
Wang, Yueyue [1 ]
Sun, Xiaofen [2 ]
Tang, Kexuan [1 ,2 ]
机构：
[1] Shanghai Jiao Tong Univ, Plant Biotechnol Res Ctr, Sch Agr & Biol, Fudan SJTU Nottingham Plant Biotechnol R&D Ctr, Shanghai 200240, Peoples R China
[2] Fudan Univ, State Key Lab Genet Engn, Sch Life Sci, Fudan SJTU Nottingham Plant Biotechnol R&D Ctr, Shanghai 200433, Peoples R China
[3] Shenyang Med Coll, Dept Biochem & Mol Biol, Shenyang 110034, Liaoning, Peoples R China
来源：
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY | 2010年 / 57卷 / 06期
关键词：
Artemisia annua; mechanical wounding; gene expression profile; artemisinin; REDUCTASE; PLANTS; L; SYNTHASE; PATHWAY; ENZYME; NADPH;
D O I：
10.1134/S102144371006018X
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Effects of mechanical wounding on gene expression involved in artemisinin biosynthesis and artemisinin production in Artemisia annua leaves were investigated. HPLC-ELSD analysis indicated that there was a remarkable enhancement of the artemisinin content in 2 h after wounding treatment, and the content reached the maximum value at 4 h (nearly 50% higher than that in the control plants). The expression profile analysis showed that many important genes (HMGR, ADS, CPR, and CYP71AV1) involved in the artemisinin biosynthetic pathway were induced in a short time after wounding treatment. This study indicates that the artemisinin biosynthesis is affected by mechanical wounding. The possible mechanism of the control of gene expression during wounding is discussed.
引用
收藏
页码：882 / 886
页数：5
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