Simultaneous Estimation of Nebivolol Hydrochloride and Hydrochlorothiazide in Tablets

A method was established for simultaneous valuation of Nebivolol hydrochloride (NBH) and Hydrochlorothiazide (HYT) from the tablet dosage form by partial simultaneous equation method, the method was found to be sensitive and accurate. The maxima of both the drugs were noted in 0.1 N hydrochloric acid. The NBH has lambda maxima at 283 nm and HYT at 270 and 320 nm. As NBH shows no absorbances at 320nm. So effective wavelengths designated were 283 nm and 270 nm for Method I, 283 nm, and 320 nm for Method II. The results of analysis for the method I exhibited greater values of standard deviation (SD), standard error of the mean (SEM), coefficient of variation (COV) and percentage range of error (within 95% confidence limit) (PROE), and thus showed less precision of the method. The results of method II analysis of marketed formulations expressively indicated fewer values. To test the accuracy and reproducibility, recovery experiments were performed. The fewer SD values depicted the novelty of the method. The method can be said to be reproducible, accurate, and precise. Thus, it was established that the method-II developed was an accurate one and can efficiently be simple, accurate, sensitive, and precise. Hence, the above two equations can be efficiently applied for the simultaneous determination of NBH and HYT in marketed formulations.