DEVELOPMENT AND VALIDATION OF AN ANALYTICAL METHOD FOR QUANTITATION OF THE DRUG BEVACIZUMAB BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

被引：0
|
作者：
de Lima Gomes, Elionai Cassiana [1 ]
Cunha Junior, Armando da Silva [1 ]
Yoshida, Maria Irene [2 ]
Jorge, Rodrigo [3 ]
机构：
[1] Univ Fed Minas Gerais, Fac Farm, BR-31270901 Belo Horizonte, MG, Brazil
[2] Univ Fed Minas Gerais, Inst Ciencias Exatas, Dept Quim, BR-31270901 Belo Horizonte, MG, Brazil
[3] Univ Sao Paulo, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP, Brazil
来源：
QUIMICA NOVA | 2012年 / 35卷 / 03期
关键词：
bevacizumab; high performance liquid chromatography; validation; COHERENCE TOMOGRAPHY FINDINGS; INTRAVITREAL INJECTION; PHARMACOKINETICS; AVASTIN(R); RABBITS;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
DEVELOPMENT AND VALIDATION OF AN ANALYTICAL METHOD FOR QUANTITATION OF THE DRUG BEVACIZUMAB BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY. In this study, an analytical method was developed and validated for quantitation of the drug bevacizumab (Avastin (R)) by high performance liquid chromatography (HPLC). The HPLC column was a BioSuite 250 (R) HR SEC, 300 x 7.8 mm x 5 mu m (Waters, USA). The mobile phase consisted of phosphate buffered saline (PBS). The results revealed that the method was specific, precise. accurate, robust and linear (r(2) = 0.998) from 5 to 75 mu g mL(-1). Therefore, this method can be used in drug release studies or in quality control ampoules of the drug.
引用
收藏
页码：608 / 611
页数：4
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