Poly(dG)-poly(dC) DNA appears shorter than poly(dA)-poly(dT) and possibly adopts an A-related conformation on a mica surface under ambient conditions

被引：11
|
作者：
Borovok, Natalia [2 ]
Molotsky, Tatiana [2 ]
Ghabboun, Jamal [1 ]
Cohen, Hezy [1 ]
Porath, Danny [1 ,3 ]
Kotlyar, Alexander [2 ,4 ]
机构：
[1] Hebrew Univ Jerusalem, Dept Phys Chem, IL-91904 Jerusalem, Israel
[2] Tel Aviv Univ, George S Wise Fac Life Sci, Dept Biochem, IL-69978 Tel Aviv, Israel
[3] Hebrew Univ Jerusalem, Ctr Nanosci & Nanotechnol, IL-91904 Jerusalem, Israel
[4] Tel Aviv Univ, Nantechnol Ctr, IL-69978 Tel Aviv, Israel
来源：
FEBS LETTERS | 2007年 / 581卷 / 30期
关键词：
atomic force microscopy (AFM); DNA imaging; Poly(dG)-poly(dC); Poly(dA)-poly(dT); A-form;
D O I：
10.1016/j.febslet.2007.11.058
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Three types of DNA: similar to 2700 bp polydeoxyguanylic olydeoxycytidylic acid [poly(dG)-poly(dC)], similar to 2700 bp polydeoxyadenylic polydeoxythymidylic acid [poly(dA)-poly(dT)] and 2686 bp linear plasmid pUC19 were deposited on a mica surface and imaged by atomic force microscopy. Contour length measurements show that the average length of poly(dG)-poly(dC) is similar to 30% shorter than that of poly(dA)-poly(dT) and the plasmid. This led us to suggest that individual poly(dG)-poly(dC) molecules are immobilized on mica under ambient conditions in a form which is likely related to the A-form of DNA in contrast to poly(dA)-poly(dT) and random sequence DNA which are immobilized in a form that is related to the DNA B-form. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：5843 / 5846
页数：4
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