Lomefloxacin Induces Oxidative Stress and Apoptosis in COLO829 Melanoma Cells

被引:35
|
作者
Beberok, Artur [1 ]
Wrzesniok, Dorota [1 ]
Szlachta, Martyna [1 ]
Rok, Jakub [1 ]
Rzepka, Zuzanna [1 ]
Respondek, Michalina [1 ]
Buszman, Ewa [1 ]
机构
[1] Med Univ Silesia, Sch Pharm, Div Lab Med, Dept Pharmaceut Chem, Jagiellonska 4, PL-41200 Sosnowiec, Poland
关键词
lomefloxacin; melanoma; oxidative stress; DNA fragmentation; apoptosis; CYCLE ARREST; IN-VITRO; FLUOROQUINOLONES; CIPROFLOXACIN; TOPOISOMERASE; GLUTATHIONE; MECHANISM; LEUKEMIA; THERAPY; GROWTH;
D O I
10.3390/ijms18102194
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Although some fluoroquinolones have been found to exert anti-tumor activity, studies on the effect of these drugs on melanoma cells are relatively rare. The aim of this study was to examine the effect of lomefloxacin on cell viability, reactive oxygen species production, redox balance, cell cycle distribution, DNA fragmentation, and apoptosis in COLO829 melanoma cells. Lomefloxacin decreases the cell viability in a dose- and time-dependent manner. For COLO829 cells treated with the drug for 24, 48, and 72 h, the values of IC50 were found to be 0.51, 0.33, and 0.25 mmol/L, respectively. The analyzed drug also altered the redox signaling pathways, as shown by intracellular reactive oxygen species overproduction and endogeneous glutathione depletion. After lomefloxacin treatment, the cells were arrested in S- and G2/M-phase, suggesting a mechanism related to topoisomerase II inhibition. DNA fragmentation was observed when the cells were exposed to increasing lomefloxacin concentrations and a prolongation of incubation time. Moreover, it was demonstrated that the drug induced mitochondrial membrane breakdown as an early hallmark of apoptosis. The obtained results provide a strong molecular basis for the pharmacologic effect underlying the potential use of lomefloxacin as a valuable agent for the treatment of melanoma in vivo.
引用
收藏
页数:18
相关论文
共 50 条
  • [41] Temozolomide induces senescence but not apoptosis in human melanoma cells
    N M Mhaidat
    X D Zhang
    J Allen
    K A Avery-Kiejda
    R J Scott
    P Hersey
    British Journal of Cancer, 2007, 97 : 1225 - 1233
  • [42] Capsaicin induces apoptosis and autophagy in human melanoma cells
    Chu, Haihan
    Li, Meng
    Wang, Xiuchun
    ONCOLOGY LETTERS, 2019, 17 (06) : 4827 - 4834
  • [43] A farnesyltransferase inhibitor manumycin A induces oxidative DNA damage and apoptosis via oxidative stress in leukemia cells.
    She, Miaorong
    Kunyuan, Guo
    Hu, Haiyan
    Niu, Xinqing
    Tu, Sanfang
    Lu, Xiaoxun
    BLOOD, 2006, 108 (11) : 170B - 170B
  • [44] Fructose induces mitochondrial dysfunction and triggers apoptosis in skeletal muscle cells by provoking oxidative stress
    Jaiswal, Natasha
    Maurya, Chandan K.
    Arha, Deepti
    Avisetti, Deepa R.
    Prathapan, Ayyappan
    Raj, Palayyan S.
    Raghu, Kozhiparambil G.
    Kalivendi, Shasi V.
    Tamrakar, Akhilesh Kumar
    APOPTOSIS, 2015, 20 (07) : 930 - 947
  • [45] Cholestasis induces apoptosis in mice cardiac cells: the possible role of nitric oxide and oxidative stress
    Shafaroodi, Hamed
    Ebrahimi, Farzad
    Moezi, Leila
    Hashemi, Mehrdad
    Doostar, Yousef
    Ghasemi, Mehdi
    Dehpour, Ahmad Reza
    LIVER INTERNATIONAL, 2010, 30 (06) : 898 - 905
  • [46] Brevilin A promotes oxidative stress and induces mitochondrial apoptosis in U87 glioblastoma cells
    Wang, Jie
    Li, Miao
    Cui, Xiaolin
    Lv, Dongyue
    Jin, Lingling
    Khan, Muhammad
    Ma, Tonghui
    ONCOTARGETS AND THERAPY, 2018, 11 : 7031 - 7040
  • [47] Auranofin induces apoptosis and necrosis in He La cells via oxidative stress and glutathione depletion
    You, Bo Ra
    Shin, Hye Rim
    Han, Bo Ram
    Kim, Suhn Hee
    Park, Woo Hyun
    MOLECULAR MEDICINE REPORTS, 2015, 11 (02) : 1428 - 1434
  • [48] Glucose withdrawal induces oxidative stress followed by apoptosis in glioblastoma cells but not in normal human astrocytes
    Jelluma, Nannette
    Yang, Xiaodong
    Stokoe, David
    Evan, Gerard I.
    Dansen, Tobias B.
    Haas-Kogan, Daphne A.
    MOLECULAR CANCER RESEARCH, 2006, 4 (05) : 319 - 330
  • [49] Zearalenone causes embryotoxicity and induces oxidative stress and apoptosis in differentiated human embryonic stem cells
    Cao, Hanwen
    Zhi, Yuan
    Xu, Haibin
    Fang, Haiqin
    Jia, Xudong
    TOXICOLOGY IN VITRO, 2019, 54 : 243 - 250
  • [50] Propyl isothiocyanate induces apoptosis in gastric cancer cells by oxidative stress via glutathione depletion
    Huang, Ling
    Cai, Chen
    Dang, Wei
    Lu, Jian-Hua
    Hu, Gang-Feng
    Gu, Jun
    ONCOLOGY LETTERS, 2019, 18 (05) : 5490 - 5498