Lomefloxacin Induces Oxidative Stress and Apoptosis in COLO829 Melanoma Cells

被引:35
|
作者
Beberok, Artur [1 ]
Wrzesniok, Dorota [1 ]
Szlachta, Martyna [1 ]
Rok, Jakub [1 ]
Rzepka, Zuzanna [1 ]
Respondek, Michalina [1 ]
Buszman, Ewa [1 ]
机构
[1] Med Univ Silesia, Sch Pharm, Div Lab Med, Dept Pharmaceut Chem, Jagiellonska 4, PL-41200 Sosnowiec, Poland
关键词
lomefloxacin; melanoma; oxidative stress; DNA fragmentation; apoptosis; CYCLE ARREST; IN-VITRO; FLUOROQUINOLONES; CIPROFLOXACIN; TOPOISOMERASE; GLUTATHIONE; MECHANISM; LEUKEMIA; THERAPY; GROWTH;
D O I
10.3390/ijms18102194
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Although some fluoroquinolones have been found to exert anti-tumor activity, studies on the effect of these drugs on melanoma cells are relatively rare. The aim of this study was to examine the effect of lomefloxacin on cell viability, reactive oxygen species production, redox balance, cell cycle distribution, DNA fragmentation, and apoptosis in COLO829 melanoma cells. Lomefloxacin decreases the cell viability in a dose- and time-dependent manner. For COLO829 cells treated with the drug for 24, 48, and 72 h, the values of IC50 were found to be 0.51, 0.33, and 0.25 mmol/L, respectively. The analyzed drug also altered the redox signaling pathways, as shown by intracellular reactive oxygen species overproduction and endogeneous glutathione depletion. After lomefloxacin treatment, the cells were arrested in S- and G2/M-phase, suggesting a mechanism related to topoisomerase II inhibition. DNA fragmentation was observed when the cells were exposed to increasing lomefloxacin concentrations and a prolongation of incubation time. Moreover, it was demonstrated that the drug induced mitochondrial membrane breakdown as an early hallmark of apoptosis. The obtained results provide a strong molecular basis for the pharmacologic effect underlying the potential use of lomefloxacin as a valuable agent for the treatment of melanoma in vivo.
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页数:18
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