Graphene Oxide Nanocolloids Induce Autophagy-Lysosome Dysfunction in Mouse Embryonic Stem Cells

被引：20
|
作者：
Wei, Min [1 ,2 ]
Fu, Zhenfa [1 ,2 ]
Wang, Che [3 ]
Zheng, Wei [1 ,2 ]
Li, Song [1 ,2 ]
Le, Weidong [1 ,2 ]
机构：
[1] Dalian Med Univ, Affiliated Hosp 1, Liaoning Prov Ctr Clin Res Neurol Dis, Dalian 116021, Peoples R China
[2] Dalian Med Univ, Affiliated Hosp 1, Liaoning Prov Key Lab Res Pathogen Mech Neurol Di, Dalian 116021, Peoples R China
[3] Liaoning Normal Univ, Sch Chem & Chem Engn, Dept Pharm, Dalian 116029, Peoples R China
来源：
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY | 2019年 / 15卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Graphene Oxide Nanocolloids; Mouse Embryonic Stem Cells; Autophagosome; Lysosome; Autophagy-Lysosome Dysfunction; POLYMERIC NANOPARTICLES; DRUG-DELIVERY; QUANTUM DOTS; IN-VITRO; NANOMATERIALS; ACCUMULATION; INHIBITION; IMPAIRMENT; GROWTH; DEATH;
D O I：
10.1166/jbn.2019.2684
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
In this study, we aimed to investigate the in vitro impacts and mechanisms of graphene oxide (GO) nanocolloids on autophagy in mouse embryonic stem cells (mESCs). Our results showed that GO nanocolloids treatment induced autophagosome accumulation in mESCs. In addition, we found that this effect was mediated by the blockade of autophagic flux rather than autophagic induction, as evidenced by the elevated autophagic substrate SQSTM1/p62 level and LC3B turn over. Moreover, our data further revealed that GO nanocolloids disrupted autophagic flux by impairing lysosomal function, including lysosomal alkalinization and lysosome membrane permeabilization. These results indicate that GO nanocolloids can block autophagic flux in mESCs via autophagy-lysosome dysfunction. Our findings may reveal the putative mechanism of GO nanocolloids-modulated autophagy and provide experimental evidence for the importance of future safety evaluation of nanomaterials.
引用
收藏
页码：340 / 351
页数：12
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