Optimisation of freeze drying conditions for purified serine protease from mango (Mangifera indica Cv. Chokanan) peel

被引:12
|
作者
Mehrnoush, Amid [3 ]
Tan, Chin Ping [2 ]
Hamed, Mirhosseini [2 ]
Aziz, Norashikin Ab. [3 ]
Ling, Tau Chuan [1 ]
机构
[1] Univ Malaya, Fac Sci, Inst Biol Sci, Kuala Lumpur 50603, Malaysia
[2] Univ Putra Malaysia, Fac Food Sci & Technol, Dept Food Technol, Upm Serdang 43400, Selangor, Malaysia
[3] Univ Putra Malaysia, Fac Engn, Dept Proc & Food Engn, Upm Serdang 43400, Selangor, Malaysia
关键词
Optimisation; Encapsulation; Serine protease; Freeze drying; Mango peel; GUM; ENCAPSULATION; TRANSITIONS; STABILITY; RELEASE; STORAGE;
D O I
10.1016/j.foodchem.2011.03.012
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
This study investigated the possible relationship between the encapsulation variables, namely serine protease content (9-50 mg/ml, X(1)), Arabic gum (0.2-10% (w/w), X(2)), maltodextrin (2-5% (w/w), X(3)) and calcium chloride (1.3-5.5% (w/w), X(4)) on the enzymatic properties of encapsulated serine protease. The study demonstrated that Arabic gum, maltodextrin and calcium chloride, as coating agents, protected serine protease from activity loss during freeze-drying. The overall optimum region resulted in a suitable freeze drying condition with a yield of 92% for the encapsulated serine protease, were obtained using 29.5 mg/ml serine protease content, 5.1% (w/w) Arabic gum, 3.5% (w/w) maltodextrin and 3.4% (w/w) calcium chloride. It was found that the interaction effect of Arabic gum and calcium chloride improved the serine protease activity, and Arabic gum was the most effective amongst the examined coating agents. Thus, Arabic gum should be considered as potential protection in freeze drying of serine protease. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码:158 / 164
页数:7
相关论文
共 46 条
  • [41] Amino acid sequence of amylase type alpha, MIamy, from ok-rong mango (Mangifera indica Linn. cv. Ok-Rong)
    Department of Biochemistry, Protein and Proteomics Research Center for Commercial and Industrial Purposes , Khon Kaen University, Khon Kaen
    40002, Thailand
    不详
    40002, Thailand
    不详
    30000, Thailand
    不详
    40002, Thailand
    [J]. Am. J. Biochem. Biotechnol., 3 (119-126):
  • [42] 7-0-Methylcyanidin 3-0-β-D-galactopyranoside, a novel anthocyanin from mango (Mangifera indica L. cv. 'Tommy Atkins') peels
    Berardini, N
    Schieber, A
    Klaiber, I
    Beifuss, U
    Carle, R
    Conrad, J
    [J]. ZEITSCHRIFT FUR NATURFORSCHUNG SECTION B-A JOURNAL OF CHEMICAL SCIENCES, 2005, 60 (07): : 801 - 804
  • [43] Identification of flavonol and xanthone glycosides from mango (Mangifera indica L. cv. "Tommy Atkins") peels by high-performance liquid chromatography-electrospray ionization mass spectrometry
    Schieber, A
    Berardini, N
    Carle, R
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2003, 51 (17) : 5006 - 5011
  • [44] Characterization of gallotannins and benzophenone derivatives from mango (Mangifera indica L. cv. 'Tommy Atkins') peels, pulp and kernels by high-performance liquid chromatography electrospray ionization mass spectrometry
    Berardini, N
    Carle, R
    Schieber, A
    [J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2004, 18 (19) : 2208 - 2216
  • [45] Identification and quantification of major phenolic compounds from mango (Mangifera indica, cv. Ataulfo) fruit by HPLC-DAD-MS/MS-ESI and their individual contribution to the antioxidant activity during ripening
    Palafox-Carlos, H.
    Yahia, E. M.
    Gonzalez-Aguilar, G. A.
    [J]. FOOD CHEMISTRY, 2012, 135 (01) : 105 - 111
  • [46] Anti-discoloration effect of phytochemicals mixture extracted from mango leaf (Mangifera indica), guava leaf (Psidium guajava']java), and green tea residue (Camellia sinensis var. sinensis cv. Chin-shin Dah-pang) on stored Nile tilapia (Oreochromis niloticus) fillets
    Kuo, I-Pei
    Liu, Ching-Shuo
    Yang, Shuenn-Der
    Hu, Yeh-Fang
    Chu, Yu-Ting
    Nan, Fan-Hua
    [J]. AQUACULTURE REPORTS, 2023, 33